Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Bacillus sp. (in: Bacteria) | |
beta-mercaptoethanol | - |
Bacillus sp. (in: Bacteria) | |
hydroxylamine | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.5 | - |
H2O2 | in 50 mM potassium phosphate buffer (pH 7.0), at 4°C | Bacillus sp. (in: Bacteria) | |
43.6 | - |
H2O2 | in 50 mM potassium phosphate buffer (pH 7.0), at 25°C | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | the active site of the enzyme contains Fe3+ | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
4 * 56000, gel filtration | Bacillus sp. (in: Bacteria) |
230000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) N2a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, and Sephacryl gel filtration | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Bacillus sp. (in: Bacteria) | O2 + H2O | - |
? | |
H2O2 | - |
Bacillus sp. (in: Bacteria) N2a | O2 + H2O | - |
? | |
additional information | BNC does not show any peroxidatic activity with 4-aminoantipyrine or pyrogallol as substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | BNC does not show any peroxidatic activity with 4-aminoantipyrine or pyrogallol as substrate | Bacillus sp. (in: Bacteria) N2a | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 56000, gel filtration | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
BNC | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | 65% residual activity after 15 min at 50°C, 54% residual activity after 30 min at 50°C, 43% residual activity after 40 min at 50°C, 12% residual activity after 15 min of preincubation at 60°C, preincubation at 65°C for 10 min results in complete loss of activity | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
149000 | - |
H2O2 | in 50 mM potassium phosphate buffer (pH 7.0), at 4°C | Bacillus sp. (in: Bacteria) | |
175000 | - |
H2O2 | in 50 mM potassium phosphate buffer (pH 7.0), at 25°C | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the active site of the enzyme contains heme | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | isoelectric focusing | - |
4.2 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0003 | - |
- |
Bacillus sp. (in: Bacteria) | hydroxylamine | |
0.0017 | - |
- |
Bacillus sp. (in: Bacteria) | azide | |
2 | - |
- |
Bacillus sp. (in: Bacteria) | beta-mercaptoethanol |