Cloned (Comment) | Organism |
---|---|
gene katA, screening of a genetic library, and DNA sequence determination and analysis, phylogenetic analysis, temperature-sensitive expression in enzyme-deficient strain Escherichia coli at 28°C, 200-300fold overexpression in a Xanthomonas campestris mutant strain XpHR | Xanthomonas campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | strong inhibition | Xanthomonas campestris | |
NaN3 | strong inhibition | Xanthomonas campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75 | - |
H2O2 | pH 7.0, 37°C | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Xanthomonas campestris | protective role of the enzyme | O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q8L2Z7 | pv. phaseoli, gene katA, enzyme KatA is the major enzyme of 2 different occurring in the organism | - |
Purification (Comment) | Organism |
---|---|
enzyme from a Xanthomonas campestris strain | Xanthomonas campestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
955 | - |
recombinant enzyme | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Xanthomonas campestris | O2 + H2O | - |
? | |
H2O2 | protective role of the enzyme | Xanthomonas campestris | O2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatA | - |
Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Xanthomonas campestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min, loss of 20% activity | Xanthomonas campestris |
55 | - |
10 min, loss of 50% activity | Xanthomonas campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
255000 | - |
H2O2 | pH 7.0, 37°C | Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | broad optimum | Xanthomonas campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | rapid drop of activity below pH 6.0 and above pH 9.0 | Xanthomonas campestris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NaN3 | pH 7.0, 37°C | Xanthomonas campestris | |
2 | - |
3-Amino-1,2,4-triazole | pH 7.0, 37°C | Xanthomonas campestris |