Crystallization (Comment) | Organism |
---|---|
enzyme in complex with wild-type cytochrome c or cytochrome c mutant DELTA10LmCytc, hanging-drop vapour diffusion method, protein in 40 mM potassium phosphate, pH 6.5, 32-33% pentaerythritol ethoxylate and 4% acetone as precipitant, X-ray diffraction structure determination and analysis at 1.84-2.29 A resolution | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics with wild-type and mutant Leishmania major cytochrome c, overview. Comparison with kinetic of the enzyme from Saccharomyces cerevisiae | Leishmania major | |
0.008 | - |
ferrocytochrome c | wild-type substrate, pH and temperature not specified in the publication | Leishmania major | |
0.01 | - |
ferrocytochrome c | DELTA10 deltion mutant substrate, pH and temperature not specified in the publication | Leishmania major | |
0.02 | - |
ferrocytochrome c | R24A substrate mutant, pH and temperature not specified in the publication | Leishmania major | |
0.04 | - |
ferrocytochrome c | K98A substrate mutant, pH and temperature not specified in the publication | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Leishmania major | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | heme | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome c + H2O2 | Leishmania major | - |
2 ferricytochrome c + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome c + H2O2 | - |
Leishmania major | 2 ferricytochrome c + 2 H2O | - |
? | |
2 ferrocytochrome c + H2O2 | activity with wild-type cytochrome c from Leishmania major and reduced activity with mutant cytochrome c R24A and K98A, no activity with cyt c mutant R24A/K98A | Leishmania major | 2 ferricytochrome c + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LmP | - |
Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Leishmania major |
General Information | Comment | Organism |
---|---|---|
additional information | cytochrome c peroxidase-cytochrome c complex: the binding interface between LmP and LmCytc has one strong and one weak ionic interaction, the Lm redox pair is more dependent on ionic interactions than on nonpolar interactions | Leishmania major |
physiological function | the parasite's peroxidase LmP helps to protect the parasite from oxidative stress. LmP is a heme peroxidase that catalyzes the peroxidation of mitochondrial cytochrome c | Leishmania major |