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Literature summary for 1.11.1.5 extracted from

  • Jasion, V.S.; Doukov, T.; Pineda, S.H.; Li, H.; Poulos, T.L.
    Crystal structure of the Leishmania major peroxidase-cytochrome c complex (2012), Proc. Natl. Acad. Sci. USA, 109, 18390-18394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with wild-type cytochrome c or cytochrome c mutant DELTA10LmCytc, hanging-drop vapour diffusion method, protein in 40 mM potassium phosphate, pH 6.5, 32-33% pentaerythritol ethoxylate and 4% acetone as precipitant, X-ray diffraction structure determination and analysis at 1.84-2.29 A resolution Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics with wild-type and mutant Leishmania major cytochrome c, overview. Comparison with kinetic of the enzyme from Saccharomyces cerevisiae Leishmania major
0.008
-
 ferrocytochrome c wild-type substrate, pH and temperature not specified in the publication Leishmania major
0.01
-
 ferrocytochrome c DELTA10 deltion mutant substrate, pH and temperature not specified in the publication Leishmania major
0.02
-
 ferrocytochrome c R24A substrate mutant, pH and temperature not specified in the publication Leishmania major
0.04
-
 ferrocytochrome c K98A substrate mutant, pH and temperature not specified in the publication Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Leishmania major 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron heme Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ferrocytochrome c + H2O2 Leishmania major
-
 2 ferricytochrome c + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferrocytochrome c + H2O2
-
 Leishmania major 2 ferricytochrome c + 2 H2O
-
 ?
2 ferrocytochrome c + H2O2 activity with wild-type cytochrome c from Leishmania major and reduced activity with mutant cytochrome c R24A and K98A, no activity with cyt c mutant R24A/K98A Leishmania major 2 ferricytochrome c + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
LmP
-
 Leishmania major

Cofactor

Cofactor Comment Organism Structure
heme
-
 Leishmania major

General Information

General Information Comment Organism
additional information cytochrome c peroxidase-cytochrome c complex: the binding interface between LmP and LmCytc has one strong and one weak ionic interaction, the Lm redox pair is more dependent on ionic interactions than on nonpolar interactions Leishmania major
physiological function the parasite's peroxidase LmP helps to protect the parasite from oxidative stress. LmP is a heme peroxidase that catalyzes the peroxidation of mitochondrial cytochrome c Leishmania major