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Literature summary for 1.11.1.5 extracted from

  • Timoteo, C.G.; Tavares, P.; Goodhew, C.F.; Duarte, L.C.; Jumel, K.; Girio, F.M.; Harding, S.; Pettigrew, G.W.; Moura, I.
    Ca2+ and the bacterial peroxidases: the cytochrome c peroxidase from Pseudomonas stutzeri (2003), J. Biol. Inorg. Chem., 8, 29-37.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM of the cation in the assay solution inhibits the oxidation of horse cytochrome c but not Pseudomonas stutzeri cytochrome c551 Pseudomonas stutzeri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ pre-incubation with the cation has no effect on activity Pseudomonas stutzeri
additional information catalytic center activity increases with ionic strength in the case of cytochrome c551, with horse heart cytochrome c, the catalytic center activity decreases exponentially Pseudomonas stutzeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
sedimentation velocity experiments, in the presence of EGTA Pseudomonas stutzeri
67000
-
sedimentation velocity experiments, untreated enzyme Pseudomonas stutzeri
80000
-
sedimentation velocity experiments, in the presence of Ca2+ Pseudomonas stutzeri
87000
-
sedimentation velocity experiments, at higher protein concentrations Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ferrocytochrome c + H2O2 Pseudomonas stutzeri
-
ferricytochrome c + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration and ion exchange chromatography Pseudomonas stutzeri

Reaction

Reaction Comment Organism Reaction ID
2 ferrocytochrome c + H2O2 + 2 H+ = 2 ferricytochrome c + 2 H2O pseudo-first-order kinetics Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + H2O2
-
Pseudomonas stutzeri ferricytochrome c + H2O
-
?

Subunits

Subunits Comment Organism
dimer ultracentrifugation, untreated enzyme Pseudomonas stutzeri
More in the presence of added Ca2+ or higher protein concentrations, the enzyme partially shifts to a higher state of aggregation, presumably tetramer Pseudomonas stutzeri