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Literature summary for 1.11.1.28 extracted from

  • Koshkin, A.; Nunn, C.M.; Djordjevic, S.; Ortiz de Montellano, P.R.
    The mechanism of Mycobacterium tuberculosis alkylhydroperoxidase AhpD as defined by mutagenesis, crystallography, and kinetics (2003), J. Biol. Chem., 278, 29502-29508 .
    View publication on PubMed
Search for more literature for 1.11.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structures of the H137F and H132Q mutants Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C130S expressed in yield comparable to that of the wild-type protein. Mutation decreases activity to less than 5% of the wild-type enzyme Mycobacterium tuberculosis
C133S expressed in yield comparable to that of the wild-type protein. Mutation completely suppresses the activity Mycobacterium tuberculosis
E118F expressed at 20% of the wild-type expression level. Mutation decreases catalytic activity Mycobacterium tuberculosis
E118Q expressed at 20% of the wild-type expression level. Mutation modestly loweres the catalytic activity Mycobacterium tuberculosis
H132F expressed in yield comparable to that of the wild-type protein. Mutation decreases the catalytic activity Mycobacterium tuberculosis
H132Q mutant tends to unfold and degrade relatively easily. Mutation decreases the catalytic activity. The H137Q mutation decreases the activity more severely than the H132Q mutation Mycobacterium tuberculosis
H137F expressed in yield comparable to that of the wild-type protein. Mutation decreases the catalytic activity Mycobacterium tuberculosis
H137Q expressed at 20% of the wild-type expression level. Mutation decreases the catalytic activity. The H137Q mutation decreases the activity more severely than the H132Q mutation Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQB5
-
-
Mycobacterium tuberculosis ATCC 25618 P9WQB5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?
a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
AhpD
-
 Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 wild-type enzyme Mycobacterium tuberculosis
7.5
-
 mutant enzymes H137F and H132F Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the enzyme provides antioxidant protection Mycobacterium tuberculosis