Literature summary for 1.11.1.27 extracted from

Pauwels, F.; Vergauwen, B.; Vanrobaeys, F.; Devreese, B.; Van Beeumen, J.J.
Purification and characterization of a chimeric enzyme from Haemophilus influenzae Rd that exhibits glutathione-dependent peroxidase activity (2003), J. Biol. Chem., 278, 16658-16666 .

Search for more literature for 1.11.1.27

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli B834(DE3)	Haemophilus influenzae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00229	-	H2O2	pH 7.8, 25°C	Haemophilus influenzae
0.2088	-	tert-butyl hydroperoxide	pH 7.8, 25°C	Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26915	-	nonreduced monomeric enzyme form, electrospray ionization mass spectrometry	Haemophilus influenzae
53216	-	oxidized dimeric enzyme form, electrospray ionization mass spectrometry	Haemophilus influenzae

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	P44758	-	-
Haemophilus influenzae ATCC 51907	P44758	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Haemophilus influenzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 glutathione + H2O2	-	Haemophilus influenzae	glutathione disulfide + 2 H2O	-	?
2 glutathione + H2O2	-	Haemophilus influenzae ATCC 51907	glutathione disulfide + 2 H2O	-	?
2 glutathione + tert-butyl hydroperoxide	-	Haemophilus influenzae	glutathione disulfide + H2O + tert-butyl alcohol	-	?
2 glutathione + tert-butyl hydroperoxide	-	Haemophilus influenzae ATCC 51907	glutathione disulfide + H2O + tert-butyl alcohol	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 26915, oxidized dimeric enzyme form, electrospray ionization mass spectrometry	Haemophilus influenzae
monomer	1 * 26915, nonreduced monomeric enzyme form, electrospray ionization mass spectrometry	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
HI0572	-	Haemophilus influenzae
PGdx	-	Haemophilus influenzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
56.7	-	tert-butyl hydroperoxide	pH 7.8, 25°C	Haemophilus influenzae
5010	-	H2O2	pH 7.8, 25°C	Haemophilus influenzae

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Release 2023.1 (January 2023)