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Literature summary for 1.11.1.24 extracted from
- Cloning and functional characterization of a typical 2-Cys peroxiredoxin from southern bluefin tuna (Thunnus maccoyii) (2010), Comp. Biochem. Physiol. B, 156, 97-106.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Prx 2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression in Escherichia coli | Thunnus maccoyii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | SBT Prx displays Michaelis-Menten kinetics with Trx but sigmoidal kinetics with H2O2 and CuOOH | Thunnus maccoyii | |
| 0.012 | - |
thioredoxin | pH 7.5, 25°C, recombinant enzyme | Thunnus maccoyii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21800 | - |
x * 21800, SDS-PAGE and sequence calculation | Thunnus maccoyii |
| 250000 | - |
recombinant enzyme, peak 1, gel filtration | Thunnus maccoyii |
| 400000 | - |
recombinant enzyme, peak 2, gel filtration | Thunnus maccoyii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thunnus maccoyii | A9QKS0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Prx 2 from Escherichia coli by nickel affinity chromatography and gel filtration | Thunnus maccoyii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thioredoxin + cumene hydroperoxide | - |
Thunnus maccoyii | thioredoxin disulfide + H2O + 2-phenylpropan-2-ol | - |
? | |
| 2 thioredoxin + H2O2 | - |
Thunnus maccoyii | thioredoxin disulfide + 2 H2O | - |
? | |
| 2 thioredoxin + t-butyl hydroperoxide | - |
Thunnus maccoyii | thioredoxin disulfide + H2O + t-butanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21800, SDS-PAGE and sequence calculation | Thunnus maccoyii |
| More | the native SBT Prx enzyme exists as a mixture of dimers, tetramers, decamers and a higher order aggregate | Thunnus maccoyii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Prx 2 | - |
Thunnus maccoyii |
| typical 2-Cys peroxiredoxin | - |
Thunnus maccoyii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Thunnus maccoyii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Thunnus maccoyii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the fish Prx 2 contains the GGLG motif associated with the sensitivity of eukaryotic typical 2-Cys Prx proteins to overoxidation and consequent inactivation by H2O2 | Thunnus maccoyii |
| physiological function | peroxiredoxins are cysteine-dependent peroxidases that function as antioxidant enzymes and also in H2O2-mediated cell signaling | Thunnus maccoyii |
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