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Literature summary for 1.11.1.24 extracted from
- Cloning and functional characterization of a peroxiredoxin 4 from yellowtail kingfish (Seriola lalandi) (2010), Comp. Biochem. Physiol. B, 156, 244-253.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene prx4, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression of enzyme versions PRX264, Prx231, and Prx197 in Escherichia coli strain BL21(DE3) | Seriola lalandi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | Seriola lalandi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | both active forms of the enzyme displayed a single-displacement reaction mechanism and typical saturable Michaelis-Menten type kinetics | Seriola lalandi | |
| 0.00098 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi |  |
| 0.0032 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 0.152 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 0.18 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 0.191 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 0.193 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thioredoxin + ROOH | Seriola lalandi | - |
thioredoxin disulfide + H2O + ROH | - |
? | |
| additional information | Seriola lalandi | shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Seriola lalandi | B9USM4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme versions PRX264, Prx231, and Prx197 from Escherichia coli strain BL21(DE3) | Seriola lalandi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thioredoxin + cumene hydroperoxide | - |
Seriola lalandi | thioredoxin disulfide + H2O + 2-phenylpropan-2-ol | - |
? | |
| 2 thioredoxin + H2O2 | - |
Seriola lalandi | thioredoxin disulfide + 2 H2O | - |
? | |
| 2 thioredoxin + ROOH | - |
Seriola lalandi | thioredoxin disulfide + H2O + ROH | - |
? | |
| 2 thioredoxin + t-butyl hydroperoxide | - |
Seriola lalandi | thioredoxin disulfide + H2O + t-butanol | - |
? | |
| additional information | shorter versions of the enzyme, Prx231 and Prx197, both exhibit thioredoxin-dependent peroxidase activity, whereas fill-length Prx264 does not | Seriola lalandi | ? | - |
? | |
| additional information | both active forms of the enzyme displayed a single-displacement reaction mechanism and typical saturable Michaelis-Menten type kinetics | Seriola lalandi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-Cys peroxiredoxin 4 | - |
Seriola lalandi |
| peroxiredoxin 4 | - |
Seriola lalandi |
| Prx 4 | - |
Seriola lalandi |
| typical 2-Cys Prx | - |
Seriola lalandi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 7.3 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 10.4 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 15.2 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 22.3 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 24.6 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 160 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 380 | - |
cumene hydroperoxide | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 580 | - |
t-butyl hydroperoxide | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
| 710 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx197 | Seriola lalandi | |
| 2500 | - |
H2O2 | pH 7.0, temperature not specified in the publication, Prx231 | Seriola lalandi | |
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