Literature summary for 1.11.1.24 extracted from

Liau, Y.; Chen, Y.; Lin, C.; Huang, J.; Lin, C.
Characterisation of 2-Cys peroxiredoxin isozyme (Prx1) from Taiwanofungus camphorata (Niu-chang-chih): Expression and enzyme properties (2010), Food Chem., 119, 154-160.

Search for more literature for 1.11.1.24

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	dithiothreitol is capable of reducing the oxidised form of the Prx1 to regenerate its activity	Taiwanofungus camphoratus
additional information	Prx1's ability to remove H2O2 is not supported by the presence of 1 mM GSH	Taiwanofungus camphoratus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C43(DE3) cells	Taiwanofungus camphoratus

General Stability

General Stability	Organism
the enzyme shows 57% activity after 40 min of incubation at 37°C with trypsin, the enzyme shows 46% activity after 20 min of incubation at 37°C with one-tenth its weight of chymotrypsin, the enzyme retains about 45% activity in 0.2 M imidazole	Taiwanofungus camphoratus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	Prx1 monomer, SDS-PAGE	Taiwanofungus camphoratus

Organic Solvent Stability

Organic Solvent	Comment	Organism
SDS	the enzyme retains 40.5% activity in 2% (w/v) SDS	Taiwanofungus camphoratus

Organism

Organism	UniProt	Comment	Textmining
Taiwanofungus camphoratus	A1KXR1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA Sepharose column chromatography	Taiwanofungus camphoratus

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit body	-	Taiwanofungus camphoratus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2O2 + reduced thioredoxin	-	Taiwanofungus camphoratus	H2O + oxidized thioredoxin	-	?

Synonyms

Synonyms	Comment	Organism
Prx1	2-Cys peroxiredoxin isozyme	Taiwanofungus camphoratus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	the enzyme retains 60% activity at 60°C for 2.5 min	Taiwanofungus camphoratus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	11	the enzyme is stable under a broad pH range from 5.0 to 11.0	Taiwanofungus camphoratus

Cofactor

Cofactor	Comment	Organism	Structure
thioredoxin	-	Taiwanofungus camphoratus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)