Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
N238S | the catalytic efficiency (Kcat/KM) of the mutant decreases to 41 and 52% for catalase and peroxidase, respectively, compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
o-Dianisidine | wild type enzyme, at pH 4.5 and 30°C | Mycobacterium tuberculosis | |
0.35 | - |
o-Dianisidine | mutant enzyme N238S, at pH 4.5 and 30°C | Mycobacterium tuberculosis | |
3.16 | - |
H2O2 | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
4.44 | - |
H2O2 | mutant enzyme N238S, at pH 7.0 and 30°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIE5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIE5 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Mycobacterium tuberculosis | O2 + H2O | - |
? | |
2 H2O2 | - |
Mycobacterium tuberculosis H37Rv | O2 + H2O | - |
? | |
isoniazid + phenol + H2O2 | - |
Mycobacterium tuberculosis | benzoquinone + ? | - |
? | |
isoniazid + phenol + H2O2 | - |
Mycobacterium tuberculosis H37Rv | benzoquinone + ? | - |
? | |
o-dianisidine + tert-butyl hydroperoxide + H2O2 | - |
Mycobacterium tuberculosis | o-dianisidin quinone diimine + ? | - |
? | |
o-dianisidine + tert-butyl hydroperoxide + H2O2 | - |
Mycobacterium tuberculosis H37Rv | o-dianisidin quinone diimine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.3 | - |
o-Dianisidine | mutant enzyme N238S, at pH 4.5 and 30°C | Mycobacterium tuberculosis | |
49 | - |
o-Dianisidine | wild type enzyme, at pH 4.5 and 30°C | Mycobacterium tuberculosis | |
227.5 | - |
H2O2 | mutant enzyme N238S, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
272.3 | - |
H2O2 | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.2 | - |
H2O2 | mutant enzyme N238S, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
86.2 | - |
H2O2 | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
96 | - |
o-Dianisidine | mutant enzyme N238S, at pH 4.5 and 30°C | Mycobacterium tuberculosis | |
199 | - |
o-Dianisidine | wild type enzyme, at pH 4.5 and 30°C | Mycobacterium tuberculosis |