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Literature summary for 1.11.1.21 extracted from

  • Kudalkar, S.N.; Njuma, O.J.; Li, Y.; Muldowney, M.; Fuanta, N.R.; Goodwin, D.C.
    A role for catalase-peroxidase large loop 2 revealed by deletion mutagenesis control of active site water and ferric enzyme reactivity (2015), Biochemistry, 54, 1648-1662 .
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E291A the mutant shows 89% peroxidase activity and 139% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
E291K the mutant shows 200% peroxidase activity and 79% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
E291K/E292A the mutant shows 222% peroxidase activity and 102% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
I290A the mutant shows 31% peroxidase activity and 93% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
I290A/Q293A the mutant shows 56% peroxidase activity and 64% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
I290V the mutant shows 35% peroxidase activity and 67% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
I290V/Q293V the mutant shows 44% peroxidase activity and 47% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
Q293A the mutant shows 59% peroxidase activity and 102% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
Q293E the mutant shows 62% peroxidase activity and 80% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis
Q293V the mutant shows 25% peroxidase activity and 44% catalase activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.028
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.037
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.05
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.06
-
 H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.06
-
 H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.07
-
 H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.071
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.078
-
 H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.08
-
 H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.08
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.081
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.087
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.089
-
 H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.09
-
 H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.09
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.1
-
 H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.13
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.16
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.21
-
 H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.31
-
 H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
0.5
-
 H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.3
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
13.6
-
 H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
15.2
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
20
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
22.5
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
23.8
-
 H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
24
-
 H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
26
-
 H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
26
-
 H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
26
-
 H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
27.8
-
 H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
28.5
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
29.3
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
29.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
32.5
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
34
-
 H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
35.3
-
 H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
38
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
39.7
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
40
-
 H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
55
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
83
-
 H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
49
-
 H2O2 mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
110
-
 H2O2 mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
140
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
160
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
200
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
210
-
 H2O2 mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
220
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
268
-
 H2O2 mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
270
-
 H2O2 wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
280
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290V/Q293V, at pH 5.0 and 23°C Mycobacterium tuberculosis
310
-
 H2O2 mutant enzyme I290A, at pH 5.0 and 23°C Mycobacterium tuberculosis
350
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme I290A/Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
350
-
 H2O2 mutant enzyme I290V, at pH 5.0 and 23°C Mycobacterium tuberculosis
370
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
380
-
 H2O2 mutant enzyme Q293A, at pH 5.0 and 23°C Mycobacterium tuberculosis
390
-
 H2O2 mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
390
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme Q293E, at pH 5.0 and 23°C Mycobacterium tuberculosis
560
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291A, at pH 5.0 and 23°C Mycobacterium tuberculosis
600
-
 H2O2 mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis
620
-
 H2O2 mutant enzyme E291K/E292A, at pH 5.0 and 23°C Mycobacterium tuberculosis
630
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, at pH 5.0 and 23°C Mycobacterium tuberculosis
1260
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme E291K, at pH 5.0 and 23°C Mycobacterium tuberculosis