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Literature summary for 1.11.1.21 extracted from
- High conformational stability of secreted eukaryotic catalase-peroxidases: answers from first crystal structure and unfolding studies (2012), J. Biol. Chem., 287, 32254-32262.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyricularia grisea |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 1.55 A resolution. Structure reveals a wrapping at the dimer interface of the N-terminal elongations from the two subunits and cysteine residues that cross-link the two subunits | Pyricularia grisea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C26A/C74A | kinetic constants similar to wild-type, decrease in melting temperature due to loss of disulfide bridge | Pyricularia grisea |
| C55A | kinetic constants similar to wild-type, decrease in melting temperature due to loss of disulfide bridge | Pyricularia grisea |
| C55A/C74A | kinetic constants similar to wild-type, decrease in melting temperature due to loss of disulfide bridge | Pyricularia grisea |
| C74A | kinetic constants similar to wild-type, decrease in melting temperature due to loss of disulfide bridge | Pyricularia grisea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.03 | - |
H2O2 | mutant C55A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea |  |
| 3.21 | - |
H2O2 | mutant C55A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 3.52 | - |
H2O2 | mutant C26A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 3.66 | - |
H2O2 | mutant C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 3.84 | - |
H2O2 | wild-type, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
2 * 85000, SDS-PAGE, recombinant protein with His-tag | Pyricularia grisea |
| 170000 | - |
PAGE | Pyricularia grisea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyricularia grisea | A4QUT2 | - |
- |
| Pyricularia grisea ATCC MYA-4617 | A4QUT2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 H2O2 | - |
Pyricularia grisea | O2 + 2 H2O | - |
? | |
| 2 H2O2 | - |
Pyricularia grisea ATCC MYA-4617 | O2 + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 85000, SDS-PAGE, recombinant protein with His-tag | Pyricularia grisea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CP 2 | - |
Pyricularia grisea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
mutants C55A, C74A, C55A/C74A, C26A/C74A, melting temperature | Pyricularia grisea |
| 60 | - |
wild-type, melting temperature | Pyricularia grisea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6446 | - |
H2O2 | wild-type, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 6729 | - |
H2O2 | mutant C55A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 6745 | - |
H2O2 | mutant C55A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 7113 | - |
H2O2 | mutant C26A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 7565 | - |
H2O2 | mutant C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Pyricularia grisea | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1680 | - |
H2O2 | wild-type, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 1880 | - |
H2O2 | mutant C26A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 1880 | - |
H2O2 | mutant C55A/C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 2020 | - |
H2O2 | mutant C74A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
| 2170 | - |
H2O2 | mutant C55A, pH not specified in the publication, temperature not specified in the publication | Pyricularia grisea | |
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