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Literature summary for 1.11.1.21 extracted from

  • Wengenack, N.L.; Uhl, J.R.; St Amand, A.L.; Tomlinson, A.J.; Benson, L.M.; Naylor, S.; Kline, B.C.; Cockerill, F.R.; Rusnak, F.
    Recombinant Mycobacterium tuberculosis KatG(S315T) is a competent catalase-peroxidase with reduced activity toward isoniazid (1997), J. Infect. Dis., 176, 722-727.
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli UM2 cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
S315T the mutant is a competent catalase-peroxidase with reduced activity toward isoniazid. The catalase activity is reduced 6fold and the peroxidase activity is decreased less than 2fold compared with the activities for the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
0.07
-
 H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
0.08
-
 isoniazid mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
0.43
-
 isoniazid wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Sephacryl S300 gel filtration, and MonoQ HR5/5 column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
isoniazid + H2O2
-
 Mycobacterium tuberculosis oxidized isoniazid + H2O
-
 ?
o-dianisidine + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis o-dianisidine quinonediimine + ?
-
 ?

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057
-
 isoniazid wild type enzyme, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
0.081
-
 isoniazid mutant enzyme S315T, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1.2
-
 o-Dianisidine mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
2
-
 o-Dianisidine wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
2100
-
 H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
5800
-
 H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme 1.1 hemes per subunit for wild type KatG Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
 o-Dianisidine mutant enzyme S315T, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
32
-
 o-Dianisidine wild type enzyme, peroxidase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
160
-
 H2O2 mutant enzyme S315T, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis
1000
-
 H2O2 wild type enzyme, catalase activity, in 50 mM sodium phosphate buffer (pH 7.5), at 37°C Mycobacterium tuberculosis