Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Synechocystis sp. | |
cyanide | - |
Synechocystis sp. | |
additional information | the enzyme is not inhibited by 3-amino-1,2,4-triazole | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
H2O2 | catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
2 * 85000, SDS-PAGE | Synechocystis sp. |
85122 | - |
2 * 85122, MALDI-TOF mass spectrometry | Synechocystis sp. |
85137 | - |
2 * 85137, calculated from amino acid sequence | Synechocystis sp. |
170000 | - |
gel filtration | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Zn2+-chelate affinity chromatography and phenyl-Sepharose column chromatography | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
crude enzyme, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C | Synechocystis sp. |
1.7 | - |
after 2.79fold purification, peroxidase activity, in 67 mM phosphate buffer, pH 7.0, at 25°C | Synechocystis sp. |
368 | - |
crude enzyme, catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |
983 | - |
after 2.67fold purification, catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Synechocystis sp. | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
guaiacol + H2O2 | - |
Synechocystis sp. | tetraguaiacol + H2O | - |
? | |
H2O2 | - |
Synechocystis sp. | O2 + H2O | - |
? | |
additional information | the recombinant protein contains high catalase activity and an appreciable peroxidase activity with o-dianisidine, guaiacol and pyrogallol, but not with NAD(P)H, ferrocytochrome c, ascorbate or glutathione as electron donors | Synechocystis sp. | ? | - |
? | |
o-dianisidine + H2O2 | - |
Synechocystis sp. | oxidized o-dianisidine + H2O | - |
? | |
pyrogallol + H2O2 | - |
Synechocystis sp. | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 85000, SDS-PAGE | Synechocystis sp. |
homodimer | 2 * 85122, MALDI-TOF mass spectrometry | Synechocystis sp. |
homodimer | 2 * 85137, calculated from amino acid sequence | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
EC 1.11.1.7 | formerly | Synechocystis sp. |
KatG | - |
Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3500 | - |
H2O2 | catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Synechocystis sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
cyanide | in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. | |
0.051 | - |
azide | in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Synechocystis sp. | isoelectric focusing | - |
5.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
710 | - |
H2O2 | catalase activity, in 67 mM phosphate buffer, pH 7.0, at 30°C | Synechocystis sp. |