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Literature summary for 1.11.1.21 extracted from

  • Hillar, A.; Peters, B.; Pauls, R.; Loboda, A.; Zhang, H.; Mauk, A.G.; Loewen, P.C.
    Modulation of the activities of catalase-peroxidase HPI of Escherichia coli by site-directed mutagenesis (2000), Biochemistry, 39, 5868-5875.
    View publication on PubMed
Search for more literature for 1.11.1.21

Protein Variants

Protein Variants Comment Organism
H106C the peroxidatic-to-catalatic ratio of the mutant is increased 125fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
H106Y the peroxidatic-to-catalatic ratio of the mutant is increased 321fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
H267Y the peroxidatic-to-catalatic ratio of the mutant is increased 36fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
R102C the peroxidatic-to-catalatic ratio of the mutant is increased 18fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
R102K the peroxidatic-to-catalatic ratio of the mutant is increased 7fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
R102L the peroxidatic-to-catalatic ratio of the mutant is increased 13fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
W105F the peroxidatic-to-catalatic ratio of the mutant is increased 2800fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli
W105L the peroxidatic-to-catalatic ratio of the mutant is increased 612fold, the heme content of the mutant is reduced relative to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Escherichia coli
cyanide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C Escherichia coli
0.055
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, at pH 6.0 and 25°C Escherichia coli
0.08
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C Escherichia coli
0.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C Escherichia coli
0.24
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C Escherichia coli
0.33
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C Escherichia coli
0.4
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C Escherichia coli
0.48
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C Escherichia coli
5.9
-
 H2O2 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
12
-
 H2O2 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
28
-
 H2O2 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
31
-
 H2O2 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
33
-
 H2O2 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
53
-
 H2O2 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
77
-
 H2O2 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
91
-
 H2O2 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
100
-
 H2O2 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli UM262
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
0.25
-
 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
0.95
-
 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
1.9
-
 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
5.9
-
 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
10
-
 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
11
-
 peroxidase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
12
-
 peroxidase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
18
-
 peroxidase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
19
-
 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
35
-
 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
63
-
 peroxidase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
85
-
 peroxidase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
85
-
 peroxidase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
660
-
 peroxidase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1300
-
 peroxidase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
1900
-
 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
1900
-
 peroxidase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli UM262 oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
H2O2
-
 Escherichia coli O2 + H2O
-
 ?
H2O2
-
 Escherichia coli UM262 O2 + H2O
-
 ?
isoniazid + H2O2
-
 Escherichia coli oxidized isoniazid + H2O
-
 ?
isoniazid + H2O2
-
 Escherichia coli UM262 oxidized isoniazid + H2O
-
 ?
o-dianisidine + H2O2
-
 Escherichia coli oxidized o-dianisidine + H2O
-
 ?
o-dianisidine + H2O2
-
 Escherichia coli UM262 oxidized o-dianisidine + H2O
-
 ?
tert-butyl hydroperoxide + H2O2
-
 Escherichia coli oxidized tert-butyl hydroperoxide + H2O
-
 ?
tert-butyl hydroperoxide + H2O2
-
 Escherichia coli UM262 oxidized tert-butyl hydroperoxide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HPI
-
 Escherichia coli
hydroperoxidase I
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0061
-
 H2O2 catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C Escherichia coli
0.089
-
 H2O2 catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C Escherichia coli
1.1
-
 H2O2 catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C Escherichia coli
1.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C Escherichia coli
1.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C Escherichia coli
2.1
-
 H2O2 catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C Escherichia coli
2.6
-
 H2O2 catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C Escherichia coli
23
-
 H2O2 catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C Escherichia coli
53.5
-
 H2O2 catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C Escherichia coli
660
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C Escherichia coli
710
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C Escherichia coli
900
-
 H2O2 catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C Escherichia coli
3700
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C Escherichia coli
5300
-
 H2O2 catalase activity, wild type enzyme, at pH 7.0 and 37°C Escherichia coli
17000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, wild type enzyme, at pH 6.0 and 25°C Escherichia coli
140000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C Escherichia coli
210000
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.008
-
 mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.008
-
 wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.01
-
 mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.012
-
 mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.04
-
 mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.055
-
 mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.065
-
 mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.075
-
 mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.09
-
 wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.095
-
 mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.18
-
 mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.19
-
 mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.21
-
 mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide
0.23
-
 mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.25
-
 mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli azide
0.39
-
 mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity Escherichia coli cyanide