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Literature summary for 1.11.1.19 extracted from

  • Chen, C.; Shrestha, R.; Jia, K.; Gao, P.F.; Geisbrecht, B.V.; Bossmann, S.H.; Shi, J.; Li, P.
    Characterization of dye-decolorizing peroxidase (DyP) from Thermomonospora curvata reveals unique catalytic properties of a-type DyPs (2015), J. Biol. Chem., 290, 23447-23463 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermomonospora curvata

Protein Variants

Protein Variants Comment Organism
D220A the catalytic efficiency of the mutant toward H2O2 drops by 22fold compared to the wild type enzyme Thermomonospora curvata
D220F the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
D220G the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
D220H the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
D220K the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
D220N the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
H312A inactive Thermomonospora curvata
H312C the catalytic efficiency of the mutant toward H2O2 drops by 88fold compared to the wild type enzyme Thermomonospora curvata
R327A inactive Thermomonospora curvata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.015
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.033
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.043
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.077
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.084
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.17
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.28
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
0.37
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.66
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
2
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
6.3
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
11
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
19
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata

Organism

Organism UniProt Comment Textmining
Thermomonospora curvata D1A807
-
-
Thermomonospora curvata DSM 43183 D1A807
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Thermomonospora curvata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Thermomonospora curvata DSM 43183 ?
-
 ?
catechol + H2O2 + H+ low activity Thermomonospora curvata ?
-
 ?
catechol + H2O2 + H+ low activity Thermomonospora curvata DSM 43183 ?
-
 ?
guaiacol + H2O2 + H+ low activity Thermomonospora curvata ?
-
 ?
guaiacol + H2O2 + H+ low activity Thermomonospora curvata DSM 43183 ?
-
 ?
guaiacylglycerol-beta-guaiacol ether + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
guaiacylglycerol-beta-guaiacol ether + H2O2 + H+
-
 Thermomonospora curvata DSM 43183 ?
-
 ?
hydroquinone + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
additional information the enzyme is inactive toward 2,6-dimethoxyphenol, 2,4-dichlorophenol, veratryl alcohol, and Reactive Black 5 Thermomonospora curvata ?
-
 ?
additional information the enzyme is inactive toward 2,6-dimethoxyphenol, 2,4-dichlorophenol, veratryl alcohol, and Reactive Black 5 Thermomonospora curvata DSM 43183 ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
Reactive Blue 4 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Thermomonospora curvata ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42200, calculated from amino acid sequence Thermomonospora curvata

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Thermomonospora curvata
DyP
-
 Thermomonospora curvata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme retains about 70% maximal activity after incubation at 60°C for 1 h Thermomonospora curvata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.36
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
0.52
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
0.58
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
0.58
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
0.59
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
24
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
27
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
29
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
31
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
41
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
110
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
260
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
280
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Thermomonospora curvata

Cofactor

Cofactor Comment Organism Structure
heme
-
 Thermomonospora curvata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.053
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
0.21
-
 H2O2 with guaiacol as cosubstrate, mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
0.79
-
 guaiacol mutant enzyme H312C, at pH 3.0 and 22°C Thermomonospora curvata
0.88
-
 guaiacol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
1.5
-
 catechol wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
2.1
-
 guaiacol mutant enzyme D220A, at pH 3.0 and 22°C Thermomonospora curvata
4.7
-
 H2O2 with guaiacol as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
4.8
-
 hydroquinone wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
650
-
 Reactive Blue 4 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
660
-
 Reactive Blue 5 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
820
-
 H2O2 with hydroquinone as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
3400
-
 H2O2 with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
7800
-
 Reactive Blue 19 wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata
17000
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 22°C Thermomonospora curvata