General Stability | Organism |
---|---|
the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively. | Streptomyces griseosporeus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 85% inhibition at 1 mM | Streptomyces griseosporeus | |
Cd2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
Co2+ | about 56% inhibition at 1 mM | Streptomyces griseosporeus | |
Hg2+ | complete inhibition at 1 mM | Streptomyces griseosporeus | |
potassium cyanide | 13% inhibition at 1 mM | Streptomyces griseosporeus | |
Sodium azide | 9% inhibition at 1 mM | Streptomyces griseosporeus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
0.458 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces griseosporeus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 116% activity at 1 mM | Streptomyces griseosporeus | |
Mn2+ | about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme | Streptomyces griseosporeus | |
additional information | Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity | Streptomyces griseosporeus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseosporeus | - |
- |
- |
Streptomyces griseosporeus SN9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
OH-40 column chromatography and UNO Q-6 column chromatography | Streptomyces griseosporeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | about 92% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
2,4,6-trichlorophenol + H2O2 + H+ | about 63% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
2,4-dichlorophenol + H2O2 + H+ | 100% activity | Streptomyces griseosporeus | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
4-chlorophenol + H2O2 + H+ | about 45% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
Acid Blue 158 + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
Cibacet Brilliant Blue BG + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? | |
guaiacol + H2O2 + H+ | about 27% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
additional information | no activity with commercial humic acid | Streptomyces griseosporeus | ? | - |
? | |
o-dianisidine + H2O2 + H+ | about 76% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
pyrogallol + H2O2 + H+ | about 75% activity compared to 2,4-dichlorophenol | Streptomyces griseosporeus | ? | - |
? | |
Remazol Brilliant Blue Reactif + H2O2 + H+ | - |
Streptomyces griseosporeus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Streptomyces griseosporeus |
Synonyms | Comment | Organism |
---|---|---|
LiP-SN | - |
Streptomyces griseosporeus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Streptomyces griseosporeus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the half-life at 60°C is 120 min | Streptomyces griseosporeus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0635 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
1.17 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces griseosporeus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C | Streptomyces griseosporeus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces griseosporeus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.567 | - |
2,4-Dichlorophenol | at pH 8.5 and 25°C | Streptomyces griseosporeus | |
2.54 | - |
o-Dianisidine | at pH 8.5 and 25°C | Streptomyces griseosporeus |