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Literature summary for 1.11.1.19 extracted from

  • Bouacem, K.; Rekik, H.; Jaouadi, N.Z.; Zenati, B.; Kourdali, S.; El Hattab, M.; Badis, A.; Annane, R.; Bejar, S.; Hacene, H.; Bouanane-Darenfed, A.; Jaouadi, B.
    Purification and characterization of two novel peroxidases from the dye-decolorizing fungus Bjerkandera adusta strain CX-9 (2017), Int. J. Biol. Macromol., 106, 636-646 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
H2O2 the maximum activity is obtained with an initial H2O2 concentration of about 0.33 mM Bjerkandera adusta

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 55% residual activity at 1 mM; 70% residual activity at 1 mM Bjerkandera adusta
Ag+ 50% residual activity at 5 mM; 82% residual activity at 5 mM Bjerkandera adusta
Ba2+ 90% residual activity at 5 mM Bjerkandera adusta
Cd2+ complete inhibition at 5 mM Bjerkandera adusta
Co2+ 66% residual activity at 5 mM Bjerkandera adusta
dithiothreitol 65% residual activity at 1 mM; 81% residual activity at 1 mM Bjerkandera adusta
Fe2+ 85% residual activity at 5 mM Bjerkandera adusta
Hg2+ complete inhibition at 5 mM Bjerkandera adusta
hydroxylamine 10% residual activity at 2 mM; 25% residual activity at 2 mM Bjerkandera adusta
L-cystine 10% residual activity at 2 mM; 25% residual activity at 2 mM Bjerkandera adusta
potassium cyanide 3% residual activity at 10 mM; complete inhibition at 10 mM Bjerkandera adusta
SDS 27% inhibition at 20 mM; 68% residual activity at 20 mM; 73% residual activity at 20 mM Bjerkandera adusta
Sodium azide 5% residual activity at 10 mM; complete inhibition at 10 mM Bjerkandera adusta
Zn2+ 88% residual activity at 5 mM Bjerkandera adusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.099
-
2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
0.099
-
2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
0.151
-
2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 118% activity at 5 mM Bjerkandera adusta
Ca2+ 110% activity at 5 mM Bjerkandera adusta
Co2+ 120% activity at 5 mM Bjerkandera adusta
Cu2+ 114% activity at 5 mM Bjerkandera adusta
Cu2+ 133% activity at 5 mM Bjerkandera adusta
Fe2+ 109% activity at 5 mM Bjerkandera adusta
K+ 125% activity at 5 mM Bjerkandera adusta
Mg2+ 180% activity at 5 mM Bjerkandera adusta
Mg2+ 180% activity at 5 mM. The addition of 5 mM of Mg2+ enhances the thermostability of the enzyme Bjerkandera adusta
Mn2+ 112% activity at 5 mM Bjerkandera adusta
Mn2+ 225% activity at 5 mM. The addition of 5 mM of Mn2+ enhances the thermostability of the enzyme Bjerkandera adusta
additional information not significantly affected by Mg2+, Ba2+, Li+, and K+ Bjerkandera adusta
additional information not significantly affected by Zn2+, Fe2+, and Li+ Bjerkandera adusta
Zn2+ 104% activity at 5 mM Bjerkandera adusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Reactive Blue 5 + H2O2 Bjerkandera adusta
-
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
hexane the enzyme retains at least 22% of the activity of incubation in the presence of 30% (v/v) hexane Bjerkandera adusta
hexane the enzyme retains at least 75% of the activity of incubation in the presence of 30% (v/v) hexane Bjerkandera adusta
isopropanol the enzyme retains at least 115% of the activity of incubation in the presence of 30% (v/v) isopropanol Bjerkandera adusta
isopropanol the enzyme retains at least 95% of the activity of incubation in the presence of 30% (v/v) isopropanol Bjerkandera adusta

Organism

Organism UniProt Comment Textmining
Bjerkandera adusta
-
-
-
Bjerkandera adusta CX-9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange column chromatography, and Sephacryl S-200 gel filtration Bjerkandera adusta
ammonium sulfate precipitation-dialysis, Sepharose column chromatography, and Sephacryl S-200 gel filtration Bjerkandera adusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.12
-
after 48fold purification, with 2,6-dimethoxyphenol as substrate, at pH 3.0 and 70°C Bjerkandera adusta
18.6
-
after 60fold purification, with 2,6-dimethoxyphenol as substrate, at pH 4.0 and 50°C Bjerkandera adusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,4-dichlorophenol Bjerkandera adusta CX-9 ?
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 82% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
?
2,4,5-trichlorophenol + H2O2 + H+ 31% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
2,4,5-trichlorophenol + H2O2 + H+ 60% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol +
-
?
2,4,5-trichlorophenol + H2O2 + H+ 31% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol + H2O
-
?
2,4,5-trichlorophenol + H2O2 + H+ 64% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol + H2O
-
?
2,4-dichlorophenol + H2O2 + H+ 100% activity Bjerkandera adusta ?
-
?
2,4-dichlorophenol + H2O2 + H+ 100% activity with 2,4-dichlorophenol, 88% activity with H2O2 Bjerkandera adusta oxidized 2,4-dichlorophenol + H2O
-
?
2,4-dichlorophenol + H2O2 + H+ 70% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4-dichlorophenol + H2O
-
?
2,6-dichlorophenol + H2O2 + H+ 69% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
2,6-dichlorophenol + H2O2 + H+ 69% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,6-dichlorophenol + H2O
-
?
2,6-dichlorophenol + H2O2 + H+ 66% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,6-dichlorophenol + H2O
-
?
2,6-dimethoxyphenol + H2O2 + H+ 75% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
2,6-dimethoxyphenol + H2O2 + H+ 75% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,6-dimethoxyphenol + H2O
-
?
2,6-dimethoxyphenol + H2O2 + H+ 100% activity with 2,6-dimethoxyphenol, 95% activity with H2O2 Bjerkandera adusta oxidized 2,6-dimethoxyphenol + H2O
-
?
2-dianisidine + H2O2 + H+ 85% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2-dianisidine + H2O
-
?
2-dianisidine + H2O2 + H+ 91% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2-dianisidine + H2O
-
?
4-chlorophenol + H2O2 + H+ 25% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 4-chlorophenol + H2O
-
?
Acid Blue 158 + H2O2 + H+
-
Bjerkandera adusta oxidized Acid Blue 158 + H2O
-
?
Acid Blue 158 + H2O2 + H+ highest decolorization activity (about 90% after 12 h) Bjerkandera adusta oxidized Acid Blue 158 + H2O
-
?
Cibacet Brilliant Blue BG + H2O2 + H+
-
Bjerkandera adusta oxidized Cibacet Brilliant Blue BG + H2O
-
?
commercial humic acid + H2O2 + H+ 81% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
coniferyl alcohol + H2O2 + H+ 97% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
coniferyl alcohol + H2O2 + H+ 97% activity compared to 2,4-dichlorophenol Bjerkandera adusta coniferaldehyde + H2O
-
?
coniferyl alcohol + H2O2 + H+ 65% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta coniferaldehyde + H2O
-
?
guaiacol + H2O2 + H+ 91% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
guaiacol + H2O2 + H+ 55% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized guaiacol + H2O
-
?
guaiacol + H2O2 + H+ 91% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized guaicol + H2O
-
?
humic acid + H2O2 + H+ 81% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized humic acid + H2O
-
?
humic acid + H2O2 + H+ 75% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized humic acid + H2O
-
?
Indigo Carmine + H2O2 + H+
-
Bjerkandera adusta oxidized Indigo Carmine + H2O
-
?
L-DOPA + H2O2 + H+ 26% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
L-DOPA + H2O2 + H+ 26% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized L-DOPA + H2O
-
?
L-DOPA + H2O2 + H+ 70% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized L-DOPA + H2O
-
?
Methyl Green + H2O2 + H+
-
Bjerkandera adusta oxidized Methyl Green + H2O
-
?
additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta ?
-
-
additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta ?
-
?
additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta CX-9 ?
-
-
additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta CX-9 ?
-
?
o-dianisidine + H2O2 + H+ 85% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
pentachlorophenol + H2O2 + H+ 10% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized pentachlorophenol + H2O
-
?
Poly R-478 + H2O2 + H+
-
Bjerkandera adusta oxidized Poly R-478 + H2O
-
?
pyrogallol + H2O2 + H+ 80% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?
pyrogallol + H2O2 + H+ 80% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized pyrogallol + H2O
-
?
pyrogallol + H2O2 + H+ 52% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized pyrogallol + H2O
-
?
Reactive Blue 5 + H2O2
-
Bjerkandera adusta phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
?
Reactive Dye Remazol Brilliant Violet 5R + H2O2 + H+
-
Bjerkandera adusta oxidized Reactive Dye Remazol Brilliant Violet 5R + H2O
-
?
Remazol Brilliant Blue Reactif + H2O2 + H+
-
Bjerkandera adusta oxidized Remazol Brilliant Blue Reactif + H2O
-
?
Remazol Brilliant Blue Reactif + H2O2 + H+ highest decolorization activity (about 90% after 12 h) Bjerkandera adusta oxidized Remazol Brilliant Blue Reactif + H2O
-
?
signayl alcohol + H2O2 + H+ 68% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized signaldehyde + H2O
-
?
signayl alcohol + H2O2 + H+ 94% activity compared to 2,4-dichlorophenol Bjerkandera adusta signaldehyde + H2O
-
?
veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta veratraldehyde + H2O
-
?
veratryl alcohol + H2O2 + H+ 72% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta veratraldehyde + H2O
-
?
veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta CX-9 veratraldehyde + H2O
-
?
veratryl alcohol + H2O2 + H+ 72% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta CX-9 veratraldehyde + H2O
-
?
veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Bjerkandera adusta
monomer 1 * 30000, SDS-PAGE Bjerkandera adusta
monomer 1 * 45221, calculated from amino acid sequence Bjerkandera adusta
monomer 1 * 30125, calculated from amino acid sequence Bjerkandera adusta

Synonyms

Synonyms Comment Organism
DyP
-
Bjerkandera adusta
LiP BA45
-
Bjerkandera adusta
MnP BA30
-
Bjerkandera adusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bjerkandera adusta
70
-
-
Bjerkandera adusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively Bjerkandera adusta
60
-
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60 °C, respectively. The half-life time of the enzyme at 40, 50, and 60°C increases to 7, 5, and 3 h in the presence 5 mM of Mg2+, respectively Bjerkandera adusta
60 80 the half-life times of the enzyme in the absence of 5 mM of Mn2+ are 8, 5, and 2 h at 60, 70, and 80°C, respectively. The half-life time of the purified peroxidase at 60, 70, and 80°C increases to 10, 7, and 4 h in the presence 5 mM of MnSO4, respectively Bjerkandera adusta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.101
-
2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
0.101
-
2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
0.207
-
2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Bjerkandera adusta
4
-
-
Bjerkandera adusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 4 the enzyme maintains 50% of its original activity at pH 2.0 and 4.0 after incubation for 9 and 5 h at 50°C, respectively Bjerkandera adusta
3 5 the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively Bjerkandera adusta

Cofactor

Cofactor Comment Organism Structure
heme
-
Bjerkandera adusta

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
at pH 4.0 and 50°C Bjerkandera adusta Ag+

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.02
-
2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
1.0235
-
2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
1.37
-
2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta