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Literature summary for 1.11.1.19 extracted from

  • Linde, D.; Pogni, R.; Canellas, M.; Lucas, F.; Guallar, V.; Baratto, M.C.; Sinicropi, A.; Saez-Jimenez, V.; Coscolin, C.; Romero, A.; Medrano, F.J.; Ruiz-Duenas, F.J.; Martinez, A.T.
    Catalytic surface radical in dye-decolorizing peroxidase a computational, spectroscopic and site-directed mutagenesis study (2015), Biochem. J., 466, 253-262 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Auricularia auricula-judae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 32.5% (w/v) PEG 4000 as precipitant Auricularia auricula-judae

Protein Variants

Protein Variants Comment Organism
G169L the mutant shows strongly increased activity compared to the wild type enzyme Auricularia auricula-judae
W377S inactive Auricularia auricula-judae
Y147F/Y337F the mutant shows reduced activity compared to the wild type enzyme Auricularia auricula-judae
Y147S the mutant shows reduced activity compared to the wild type enzyme Auricularia auricula-judae
Y337S the mutant shows about wild type activity Auricularia auricula-judae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
0.0156
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
0.09
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
0.121
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
0.703
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
2.75
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
7.36
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae

Organism

Organism UniProt Comment Textmining
Auricularia auricula-judae I2DBY1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Auricularia auricula-judae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Auricularia auricula-judae ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
veratryl alcohol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Auricularia auricula-judae
DyP-I
-
 Auricularia auricula-judae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae
0.7
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
4.8
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
120
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
171
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
224
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
224
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae

Cofactor

Cofactor Comment Organism Structure
heme
-
 Auricularia auricula-judae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.032
-
 veratryl alcohol mutant enzyme W377S, at pH 2.5 and 25°C Auricularia auricula-judae
0.096
-
 veratryl alcohol wild type enzyme, at pH 2.5 and 25°C Auricularia auricula-judae
62
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant enzyme W377S, at pH 3.0 and 25°C Auricularia auricula-judae
68
-
 Reactive Blue 5 mutant enzyme W377S, at pH 3.5 and 25°C Auricularia auricula-judae
200
-
 2,6-dimethoxyphenol wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
310
-
 Reactive Blue 5 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae
1850
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild type enzyme, at pH 3.0 and 25°C Auricularia auricula-judae
2460
-
 Reactive Blue 19 wild type enzyme, at pH 3.5 and 25°C Auricularia auricula-judae