Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 10 mM, 33% residual activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
Reactive Blue 5 | pH 3.5, 30°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 10 mM, 117% of initial activity | Pseudomonas aeruginosa | |
Co2+ | 10 mM, 115% of initial activity | Pseudomonas aeruginosa | |
Zn2+ | 10 mM, 122% of initial activity | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Pseudomonas aeruginosa |
64000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | D5LRR6 | - |
- |
Pseudomonas aeruginosa PKE117 | D5LRR6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 + H+ | 18.3% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | 18.3% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa PKE117 | ? | - |
? | |
Reactive Black 5 + H2O2 | 16.4% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Black 5 + H2O2 | 16.4% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa PKE117 | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 19 + H2O2 | 19% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Blue 19 + H2O2 | 19% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa PKE117 | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Blue 5 + H2O2 | - |
Pseudomonas aeruginosa | phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate | - |
? | |
Reactive Blue 5 + H2O2 | - |
Pseudomonas aeruginosa PKE117 | phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate | - |
? | |
Vat Blue 6 + H2O2 | 5.1% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa | oxidized Vat Blue 6 + H2O | - |
? | |
Vat Blue 6 + H2O2 | 5.1% of the activity with Reactive Blue 5 | Pseudomonas aeruginosa PKE117 | oxidized Vat Blue 6 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
DyP | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
4 h, 83% residual activity | Pseudomonas aeruginosa |
70 | - |
1 h, more than 70% residual activity | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0235 | - |
Reactive Blue 5 | pH 3.5, 30°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
rapid loss of activity below | Pseudomonas aeruginosa |
6.5 | 7 | 30 min, stable | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme displays typical heme absorbance of Soret band, with an Rz value of 1.18 | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | calculated | - |
4.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.219 | - |
Reactive Blue 5 | pH 3.5, 30°C | Pseudomonas aeruginosa |