Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and in Aspergillus oryzae strain RD005 | Thanatephorus cucumeris |
Protein Variants | Comment | Organism |
---|---|---|
H164A | the specific activity of the purified mutant is 99.8% lower than that of recombinant DyP expressed in Escherichia coli | Thanatephorus cucumeris |
H166A | the specific activity of the purified mutant is 95% lower than that of recombinant DyP expressed in Escherichia coli | Thanatephorus cucumeris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Thanatephorus cucumeris |
47460 | - |
recombinant enzyme expressed in Escherichia coli, deduced from amino acid sequence | Thanatephorus cucumeris |
50000 | - |
recombinant enzyme expressed in Escherichia coli, gel filtration | Thanatephorus cucumeris |
58000 | - |
recombinant enzyme expressed in Aspergillus oryzae, gel filtration | Thanatephorus cucumeris |
60000 | - |
native enzyme, gel filtration | Thanatephorus cucumeris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thanatephorus cucumeris | - |
strain Dec 1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thanatephorus cucumeris |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, QAE-Toyopearl column chromatography, Mono Q column chromatography, Poros PE column chromatography, and Superdex 75 gel filtration | Thanatephorus cucumeris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.058 | - |
recombinant enzyme from crude extract, at 30°C, pH 3.2 | Thanatephorus cucumeris |
834 | - |
recombinant enzyme after 14385fold purification, at 30°C, pH 3.2 | Thanatephorus cucumeris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 + H+ | native DyP shows 9.3% activity, recombinant enzyme expressed in Escherichia coli shows 9.0% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 8.1% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | ? | - |
? | |
guaiacol + H2O2 + H+ | native DyP shows 9.2% activity, recombinant enzyme expressed in Escherichia coli shows 10% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 6.0% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | ? | - |
? | |
additional information | native and recombinant DyPs do not oxidize veratryl alcohol | Thanatephorus cucumeris | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | native DyP shows 1.0% activity, recombinant enzyme expressed in Escherichia coli shows 0.9% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 1.0% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 114 + H2O2 + H+ | native DyP shows 4.4% activity, recombinant enzyme expressed in Escherichia coli shows 2.4% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 2.2% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 114 + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | native DyP shows 119% activity, recombinant enzyme expressed in Escherichia coli shows 102% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 101% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 21 + H2O2 + H+ | native DyP shows 26% activity, recombinant enzyme expressed in Escherichia coli shows 32% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 25% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Blue 21 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | native DyP and recombinant enzymes expressed in Escherichia coli and Aspergillus oryzae show 100% activity | Thanatephorus cucumeris | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Orange 13 + H2O2 + H+ | native DyP shows 0.22% activity compared to Reactive Blue 5, recombinant enzymes expressed in Escherichia coli or in Aspergillus oryzae show no activity with Reactive Orange 13 | Thanatephorus cucumeris | oxidized Reactive Orange 13 + H2O | - |
? | |
Reactive Red 120 + H2O2 + H+ | native DyP shows 0.96% activity, recombinant enzyme expressed in Escherichia coli shows 0.61% activity compared to Reactive Blue 5, and recombinant enzyme expressed in Aspergillus oryzae shows no activity with Reactive Red 120 | Thanatephorus cucumeris | oxidized Reactive Red 120 + H2O | - |
? | |
Reactive Red 33 + H2O2 + H+ | native DyP shows 2.3 activity, recombinant enzyme expressed in Escherichia coli shows 1.8% activity, and recombinant enzyme expressed in Aspergillus oryzae shows 2.2% activity compared to Reactive Blue 5 | Thanatephorus cucumeris | oxidized Reactive Red 33 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Thanatephorus cucumeris |
DyP | - |
Thanatephorus cucumeris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | recombinant DyP expressed in Escherichia coli does not lose its activity at 30°C and 40°C. The activity is loweres slightly to 83% of full activity after heating 1 h at 50°C. In contrast, the activity drastically declines at 60°C and 70°C. The residual activity is 4% and 10% of full activity after heating 2 h at 60°C and 15 min at 70°C, respectively. The activity of native DyP after 1 h heating at 60°C is 92% of full activity whereas that of recombinant DyPs expressed in Escherichia coli and Aspergillus oryzae are only 42%, and 44%, respectively | Thanatephorus cucumeris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Thanatephorus cucumeris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Thanatephorus cucumeris |