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Literature summary for 1.11.1.19 extracted from

  • Liers, C.; Bobeth, C.; Pecyna, M.; Ullrich, R.; Hofrichter, M.
    DyP-like peroxidases of the jelly fungus Auricularia auricula-judae oxidize nonphenolic lignin model compounds and high-redox potential dyes (2010), Appl. Microbiol. Biotechnol., 85, 1869-1879.
    View publication on PubMed
Search for more literature for 1.11.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 Reactive Black 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.005
-
 H2O2 manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.007
-
 Reactive Black 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.01
-
 H2O2 manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.015
-
 Reactive Blue 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.02
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.02
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.023
-
 Reactive Blue 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.023
-
 2,6-dimethoxyphenol manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.027
-
 2,6-dimethoxyphenol manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
1.779
-
 veratryl alcohol manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
2.89
-
 veratryl alcohol manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme shows manganese-independent peroxidase activity Auricularia auricula-judae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 manganese-independent peroxidase II, SDS-PAGE Auricularia auricula-judae
51000
-
 manganese-independent peroxidase I, SDS-PAGE Auricularia auricula-judae

Organism

Organism UniProt Comment Textmining
Auricularia auricula-judae
-
 strain 11326
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, Mono Q column chromatography, Mono S column chromatography, and Mono P column chromatography Auricularia auricula-judae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.4
-
 culture liquid, at pH 3.0 and 20°C Auricularia auricula-judae
347.8
-
 manganese-independent peroxidase II after 12.2fold purification, at pH 3.0 and 20°C Auricularia auricula-judae
469
-
 manganese-independent peroxidase I after 16.5fold purification, at pH 3.0 and 20°C Auricularia auricula-judae

Storage Stability

Storage Stability Organism
20°C, manganese-independent peroxidase I in 50 mM sodium tartrate buffer pH 2.5, 24 h, 20% loss of activity Auricularia auricula-judae
20°C, manganese-independent peroxidase II in 50 mM sodium tartrate buffer pH 2.5, 24 h, 75% loss of activity Auricularia auricula-judae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Auricularia auricula-judae ?
-
 ?
adlerol + H2O2 + H+ i.e 1-(3,4-dimethoxyphenyl)-1-oxo-2-(2-methoxyphenoxy)-1,3-dihydroxy-propane, maximum oxidation activity at pH 2.5 Auricularia auricula-judae ?
-
 ?
additional information Mn2+ is not oxidized Auricularia auricula-judae ?
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Auricularia auricula-judae oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 5 + H2O2 + H+
-
 Auricularia auricula-judae oxidized Reactive Blue 5 + H2O
-
 ?
veratryl alcohol + H2O2 + H+ maximum oxidation activity at pH 1.4 Auricularia auricula-judae veratraldehyde + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, manganese-independent peroxidase II, SDS-PAGE Auricularia auricula-judae
monomer 1 * 51000, manganese-independent peroxidase I, SDS-PAGE Auricularia auricula-judae

Synonyms

Synonyms Comment Organism
manganese-independent peroxidase I
-
 Auricularia auricula-judae
manganese-independent peroxidase II
-
 Auricularia auricula-judae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.62
-
 veratryl alcohol manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.86
-
 veratryl alcohol manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
3
-
 Reactive Black 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
3
-
 Reactive Black 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
89
-
 2,6-dimethoxyphenol manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
90
-
 2,6-dimethoxyphenol manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
114
-
 Reactive Blue 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
238
-
 H2O2 manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
256
-
 Reactive Blue 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
268
-
 H2O2 manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
322
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
368
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4
-
 maximum activity at pH 1.4 using veratryl alcohol as substrate Auricularia auricula-judae

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 at pH 2.5, manganese-independent peroxidase I is quite stable and does not lose any activity within 4 h, while manganese-independent peroxidase II loses about 40% of its activity within the same time Auricularia auricula-judae

Cofactor

Cofactor Comment Organism Structure
heme heme-containing enzyme Auricularia auricula-judae

pI Value

Organism Comment pI Value Maximum pI Value
Auricularia auricula-judae manganese-independent peroxidase II, isoelectric focusing
-
 4.1
Auricularia auricula-judae manganese-independent peroxidase I, isoelectric focusing
-
 4.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.22
-
 veratryl alcohol manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
0.48
-
 veratryl alcohol manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
410
-
 Reactive Black 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
570
-
 Reactive Black 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
3300
-
 2,6-dimethoxyphenol manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
3900
-
 2,6-dimethoxyphenol manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
5000
-
 Reactive Blue 5 manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
17000
-
 Reactive Blue 5 manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
27000
-
 H2O2 manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae
48000
-
 H2O2 manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C Auricularia auricula-judae