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Literature summary for 1.11.1.18 extracted from

  • Carter, J.N.; Beatty, K.E.; Simpson, M.T.; Butler, A.
    Reactivity of recombinant and mutant vanadium bromoperoxidase from the red alga Corallina officinalis (2002), J. Inorg. Biochem., 91, 59-69.
    View publication on PubMed
Search for more literature for 1.11.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corallina officinalis

Protein Variants

Protein Variants Comment Organism
H480A results in the loss of the ability to efficiently oxidize bromide, but retains the ability to oxidize iodide Corallina officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 Br- pH 6.5, recombinant enzyme Corallina officinalis
0.0013
-
 Br- pH 6.5, native enzyme Corallina officinalis
0.017
-
 H2O2 pH 6.5, recombinant enzyme Corallina officinalis
0.027
-
 H2O2 pH 6.5, native enzyme Corallina officinalis
0.2
-
 H2O2 pH 6.5, mutant enzyme H480A Corallina officinalis
1.8
-
 I- recombinant enzyme Corallina officinalis
33
-
 I- mutant enzyme H480A Corallina officinalis

Organism

Organism UniProt Comment Textmining
Corallina officinalis Q8LLW7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corallina officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Corallina officinalis ?
-
 ?
I- + H2O2
-
 Corallina officinalis triiodide + ?
-
 ?

Synonyms

Synonyms Comment Organism
CVBPO
-
 Corallina officinalis
vanadium bromoperoxidase
-
 Corallina officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 maximum bromoperoxidase activity of mutant H480A under conditions of high substrate concentrations (5 mM H2O2 and 100 mM Br-), however with reduced specific activity as compared to recombinant wild-type enzyme Corallina officinalis
6
-
 optimal bromoperoxidase activity of recombinant wild-type enzyme using low substrate concentrations (0.5 mM H2O2, 5 mM Br-) and high substrate concentrations (5 mM H2O2, 100 mM Br-) Corallina officinalis

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 35% of maximal activity, pH 7.0: about 25% of maximal activity Corallina officinalis