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Literature summary for 1.11.1.18 extracted from

  • Zeiner, R.; van Pee, K.H.; Lingens, F.
    Purification and partial characterization of multiple bromoperoxidases from Streptomyces griseus (1988), J. Gen. Microbiol., 134, 3141-3149.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
1,1-dimethyl-4-chloro-3,5-cyclohexanedione nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus
0.0079
-
1,1-dimethyl-4-chloro-3,5-cyclohexanedione nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
6.4
-
Br- nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus
95
-
H2O2 nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus
160
-
Br- nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
430
-
H2O2 nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus
43000
-
2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b Streptomyces griseus
70000
-
gel filtration, nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
90000
-
gel filtration, nonhaem-type bromoperoxidase BPO 1b Streptomyces griseus
90000
-
gel filtration, nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
strain Tü 6
-
Streptomyces griseus Tu6
-
strain Tü 6
-

Purification (Commentary)

Purification (Comment) Organism
nonhaem-type bromoperoxidase BPO1a Streptomyces griseus
nonhaem-type bromoperoxidase BPO1b Streptomyces griseus
nonhaem-type bromoperoxidase BPO3 Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces griseus
1.1
-
nonhaem-type bromoperoxidase BPO3 Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Streptomyces griseus ?
-
?
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Streptomyces griseus Tu6 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 3400, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
dimer 2 * 43000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 1b Streptomyces griseus
trimer 3 * 31000, SDS-PAGE, nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus

Synonyms

Synonyms Comment Organism
nonhaem-type bromoperoxidase BPO 1a
-
Streptomyces griseus
nonhaem-type bromoperoxidase BPO 1b
-
Streptomyces griseus
nonhaem-type bromoperoxidase BPO 3
-
Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
60
-
nonhaem-type bromoperoxidase BPO3 Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
50 min, complete inactivation, nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
60
-
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 1b Streptomyces griseus
60
-
50 min, no loss of activity, nonhaem-type bromoperoxidase BPO 3 Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
nonhaem-type bromoperoxidase BPO 1a Streptomyces griseus
4.5
-
nonhaem-type bromoperoxidase BPO3, at low concentrations of H2O2 (1.6 mM) the pH-optimum shifts from pH 4.5 to pH 4.0 Streptomyces griseus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces griseus isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 3
-
3.6
Streptomyces griseus isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1a
-
4.6
Streptomyces griseus isoelectric focusing, pH-range 3.5-9.5, nonhaem-type bromoperoxidase BPO 1b
-
4.7