Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL(DE3) | Marichromatium gracile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27430 | - |
calculated from the deduced amino acid sequence | Marichromatium gracile |
27500 | - |
chimeric enzyme composed of one N-terminal peroxidoxin-like domain followed by a glutaredoxin-like C-terminus, SDS-PAGE | Marichromatium gracile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marichromatium gracile | - |
- |
- |
Marichromatium gracile DSM 1712 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of the Prx/Grx-containing crude extract, centrifugation, ammonium sulfate precipitation, butyl-Sepharose HR 16/10 column, dialysis, ResourceQ column | Marichromatium gracile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione amide + H2O2 | similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity | Marichromatium gracile | glutathione amide disulfide + H2O | - |
? | |
glutathione amide + H2O2 | similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity | Marichromatium gracile DSM 1712 | glutathione amide disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
garB | - |
Marichromatium gracile |
More | glutathione amide reductase, cf. EC 1.8.1.16 | Marichromatium gracile |
Prx-containing peroxidase | - |
Marichromatium gracile |
Prx/Grx chimeric enzyme | peroxidoxin/glutaredoxin chimeric enzyme | Marichromatium gracile |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Marichromatium gracile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Marichromatium gracile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Marichromatium gracile |
General Information | Comment | Organism |
---|---|---|
physiological function | garB encodes the central enzyme in glutathione amide cycling. The enzyme is the first example of a prokaryotic low molecular mass thiol-dependent peroxidase. The hydroperoxide-dependent Prx/Grx-catalyzed oxidation of glutathione amide is established in vitro, and as to be expected, the reaction is fueled by NADH via glutathione amide redox recycling | Marichromatium gracile |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |