Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli W3110 cells | Pleurotus eryngii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M sodium MES buffer at pH 6.5, 25% (w/v) PEG 4000 and 0.2 M MgCl2 | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | - |
- |
Purification (Comment) | Organism |
---|---|
Resource-Q column chromatography | Pleurotus eryngii |
Renatured (Comment) | Organism |
---|---|
using 0.16 M urea, 0.02 mM hemin, 5 mM CaCl2, 0.5 mM GSSG, 0.1 mM dithiothreitol, and 0.1 mg/ml protein in 20 mM Tris-HCl at pH 9.5 | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Pleurotus eryngii | ? | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | - |
? | |
Mn2+ + H2O2 + Reactive Black 5 | - |
Pleurotus eryngii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Vpl2 | isoform | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pleurotus eryngii |