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Literature summary for 1.11.1.16 extracted from

  • Saez-Jimenez, V.; Fernandez-Fueyo, E.; Medrano, F.J.; Romero, A.; Martinez, A.T.; Ruiz-Duenas, F.J.
    Improving the pH-stability of versatile peroxidase by comparative structural analysis with a naturally-stable manganese peroxidase (2015), PLoS ONE, 10, e0140984 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli W3110 cells Pleurotus eryngii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium MES buffer at pH 6.5, 25% (w/v) PEG 4000 and 0.2 M MgCl2 Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource-Q column chromatography Pleurotus eryngii

Renatured (Commentary)

Renatured (Comment) Organism
using 0.16 M urea, 0.02 mM hemin, 5 mM CaCl2, 0.5 mM GSSG, 0.1 mM dithiothreitol, and 0.1 mg/ml protein in 20 mM Tris-HCl at pH 9.5 Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
Pleurotus eryngii ?
-
?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O
-
?
Mn2+ + H2O2 + Reactive Black 5
-
Pleurotus eryngii ?
-
?

Synonyms

Synonyms Comment Organism
Vpl2 isoform Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Pleurotus eryngii