Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.16 extracted from

  • Knop, D.; Levinson, D.; Makovitzki, A.; Agami, A.; Lerer, E.; Mimran, A.; Yarden, O.; Hadar, Y.
    Limits of versatility of versatile peroxidase (2016), Appl. Environ. Microbiol., 82, 4070-4080 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 H2O2 at pH 5.0 and 25°C Pleurotus ostreatus
0.0248
-
 Reactive Black 5 at pH 3.5 and 25°C Pleurotus ostreatus
0.0358
-
 Orange II at pH 3.0 and 25°C Pleurotus ostreatus
0.171
-
 Mn2+ at pH 5.0 and 25°C Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus PC9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q column chromatography, and Mono Q column chromatography Pleurotus ostreatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.86
-
 unpurified enzyme, with Mn2+ as substrate, at pH 5.0 and 25°C Pleurotus ostreatus
61.48
-
 after 5.66fold purification, with Mn2+ as substrate, at pH 5.0 and 25°C Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H+ + H2O2
-
 Pleurotus ostreatus Mn3+ + H2O
-
 ?
Mn2+ + H+ + H2O2
-
 Pleurotus ostreatus PC9 Mn3+ + H2O
-
 ?
Orange II + H+ + H2O2
-
 Pleurotus ostreatus ?
-
 ?
Orange II + H+ + H2O2
-
 Pleurotus ostreatus PC9 ?
-
 ?
Phenol Red + H+ + H2O2
-
 Pleurotus ostreatus ?
-
 ?
Phenol Red + H+ + H2O2
-
 Pleurotus ostreatus PC9 ?
-
 ?
Reactive Black 5 + H+ + H2O2
-
 Pleurotus ostreatus ?
-
 ?
Reactive Black 5 + H+ + H2O2
-
 Pleurotus ostreatus PC9 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Pleurotus ostreatus

Synonyms

Synonyms Comment Organism
VP1
-
 Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 Residual activity of roughly 100% is found after incubation at 25 to 55°C with all three substrates. Stability decreases gradually at temperatures from 60 to 70°C Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 H2O2 at pH 5.0 and 25°C Pleurotus ostreatus
0.83
-
 Orange II at pH 3.0 and 25°C Pleurotus ostreatus
6.2
-
 Mn2+ at pH 5.0 and 25°C Pleurotus ostreatus
54
-
 Reactive Black 5 at pH 3.5 and 25°C Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 pH optimum for Orange II oxidation Pleurotus ostreatus
3.5
-
 pH optimum for Reactive Black 5 oxidation Pleurotus ostreatus
5
-
 pH optimum for Mn2+ oxidation Pleurotus ostreatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.7
-
 Orange II at pH 3.0 and 25°C Pleurotus ostreatus
80.3
-
 Mn2+ at pH 5.0 and 25°C Pleurotus ostreatus
2224.4
-
 Reactive Black 5 at pH 3.5 and 25°C Pleurotus ostreatus
5961
-
 H2O2 at pH 5.0 and 25°C Pleurotus ostreatus