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Literature summary for 1.11.1.16 extracted from

  • Gonzalez-Perez, D.; Garcia-Ruiz, E.; Ruiz-Duenas, F.; Martinez, A.; Alcalde, M.
    Structural determinants of oxidative stabilization in an evolved versatile peroxidase (2014), ACS Catal., 4, 3891-3901 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
Reactive Black 5 at pH 3.0 and 25°C Pleurotus eryngii
0.045
-
Mn2+ at pH 5.0 and 25°C Pleurotus eryngii
0.54
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.0 and 25°C Pleurotus eryngii
32
-
2,6-dimethoxyphenol at pH 3.0 and 25°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H+ + H2O2
-
Pleurotus eryngii ?
-
?
2,6-dimethoxyphenol + H+ + H2O2
-
Pleurotus eryngii ?
-
?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O
-
?
Reactive Black 5 + H+ + H2O2
-
Pleurotus eryngii ?
-
?

Synonyms

Synonyms Comment Organism
Vpl2 isoform Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.8
-
Reactive Black 5 at pH 3.0 and 25°C Pleurotus eryngii
54
-
Mn2+ at pH 5.0 and 25°C Pleurotus eryngii
98
-
2,6-dimethoxyphenol at pH 3.0 and 25°C Pleurotus eryngii
220
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.0 and 25°C Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1
-
2,6-dimethoxyphenol at pH 3.0 and 25°C Pleurotus eryngii
410
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 3.0 and 25°C Pleurotus eryngii
1190
-
Mn2+ at pH 5.0 and 25°C Pleurotus eryngii
1670
-
Reactive Black 5 at pH 3.0 and 25°C Pleurotus eryngii