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Literature summary for 1.11.1.16 extracted from

  • Singh, R.; Grigg, J.C.; Armstrong, Z.; Murphy, M.E.; Eltis, L.D.
    Distal heme pocket residues of B-type dye-decolorizing peroxidase: arginine but not aspartate is essential for peroxidase activity (2012), J. Biol. Chem., 287, 10623-10630.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.16

Crystallization (Commentary)

Crystallization (Comment) Organism
structural changes in the mutants D153H, D153A, R244L, N246H, N246A, D153A/N246A are confined to the distal heme environment Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
D153A mutation minimally affects the second order rate constant for Compound I formation and the specificity constant for H2O2, but substitution dramatically reduces the stability of Compound I Rhodococcus jostii
D153A/N246A mutation reduces the second order rate constant for Compound I formation and the specificity constant for H2O2 less than 30fold, substitution dramatically reduces the stability of Compound I Rhodococcus jostii
D153H mutant is more than an order of magnitude less reactive with H2O2 than wild-type Rhodococcus jostii
N246A mutation inimally affects the second order rate constant for Compound I formation and the specificity constant for H2O2, but substitution dramatically reduces the stability of Compound I Rhodococcus jostii
N246H no detectable peroxidase activity Rhodococcus jostii
R244L mutation abolishes the peroxidase activity, and heme iron of the mutant shows a pH-dependent transition from high spin pH 5 to low spin pH 8.5 Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 H2O2 mutant N246A, pH 5.5, 25°C Rhodococcus jostii
0.027
-
 H2O2 wild-type, pH 5.5, 25°C Rhodococcus jostii
0.087
-
 H2O2 mutant D153H, pH 5.5, 25°C Rhodococcus jostii
0.46
-
 H2O2 mutant D153A, pH 5.5, 25°C Rhodococcus jostii
5
-
 H2O2 mutant D153A/N246A, pH 5.5, 25°C Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SE24
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Rhodococcus jostii ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
B-type dye-decolorizing peroxidase
-
 Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 H2O2 mutant D153H, pH 5.5, 25°C Rhodococcus jostii
3.2
-
 H2O2 wild-type, pH 5.5, 25°C Rhodococcus jostii
5.4
-
 H2O2 mutant N246A, pH 5.5, 25°C Rhodococcus jostii
19
-
 H2O2 mutant D153A/N246A, pH 5.5, 25°C Rhodococcus jostii
51
-
 H2O2 mutant D153A, pH 5.5, 25°C Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
heme
-
 Rhodococcus jostii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 H2O2 mutant D153A/N246A, pH 5.5, 25°C Rhodococcus jostii
20
-
 H2O2 mutant D153H, pH 5.5, 25°C Rhodococcus jostii
110
-
 H2O2 mutant D153A, pH 5.5, 25°C Rhodococcus jostii
120
-
 H2O2 wild-type, pH 5.5, 25°C Rhodococcus jostii
360
-
 H2O2 mutant N246A, pH 5.5, 25°C Rhodococcus jostii