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Literature summary for 1.11.1.16 extracted from

  • Bao, X.; Liu, A.; Lu, X.; Li, J.J.
    Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli (2012), Biotechnol. Lett., 34, 1537-1543.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli fused to a thioredoxin-hexahistidine tag. Activity of the enzyme increases after removing the tag Pleurotus eryngii

Inhibitors

Inhibitors Comment Organism Structure
H2O2 6.4 mM, 5% loss of activity Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C Pleurotus eryngii
0.0048
-
Reactive Black 5 pH 3.5, 25°C Pleurotus eryngii
0.078
-
Mn2+ pH 5.0, 25°C Pleurotus eryngii
4.09
-
veratryl alcohol pH 3.0, 25°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753 isoform Vpl2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pleurotus eryngii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
substrate Reactive Black 5, pH 3.5, 25°C Pleurotus eryngii
7.2
-
substrate veratryl alcohol, pH 3.0, 25°C Pleurotus eryngii
8.8
-
substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.5, 25°C Pleurotus eryngii
194
-
substrate Mn2+, pH 5.0, 25°C Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn2+ + 2 H+ + H2O2
-
Pleurotus eryngii 2 Mn3+ + 2 H2O
-
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
Pleurotus eryngii ? + H2O
-
?
Reactive Black 5 + H2O2
-
Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Pleurotus eryngii veratraldehyde + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
Reactive Black 5 pH 3.5, 25°C Pleurotus eryngii
5.4
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C Pleurotus eryngii
6.4
-
veratryl alcohol pH 3.0, 25°C Pleurotus eryngii
138
-
Mn2+ pH 5.0, 25°C Pleurotus eryngii