Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli fused to a thioredoxin-hexahistidine tag. Activity of the enzyme increases after removing the tag | Pleurotus eryngii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | 6.4 mM, 5% loss of activity | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C | Pleurotus eryngii | |
0.0048 | - |
Reactive Black 5 | pH 3.5, 25°C | Pleurotus eryngii | |
0.078 | - |
Mn2+ | pH 5.0, 25°C | Pleurotus eryngii | |
4.09 | - |
veratryl alcohol | pH 3.0, 25°C | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | isoform Vpl2 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pleurotus eryngii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
substrate Reactive Black 5, pH 3.5, 25°C | Pleurotus eryngii |
7.2 | - |
substrate veratryl alcohol, pH 3.0, 25°C | Pleurotus eryngii |
8.8 | - |
substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.5, 25°C | Pleurotus eryngii |
194 | - |
substrate Mn2+, pH 5.0, 25°C | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn2+ + 2 H+ + H2O2 | - |
Pleurotus eryngii | 2 Mn3+ + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus eryngii | ? + H2O | - |
? | |
Reactive Black 5 + H2O2 | - |
Pleurotus eryngii | oxidized Reactive Black 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
Reactive Black 5 | pH 3.5, 25°C | Pleurotus eryngii | |
5.4 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C | Pleurotus eryngii | |
6.4 | - |
veratryl alcohol | pH 3.0, 25°C | Pleurotus eryngii | |
138 | - |
Mn2+ | pH 5.0, 25°C | Pleurotus eryngii |