Application | Comment | Organism |
---|---|---|
degradation | versatile peroxidase presents particular interest due to its catalytic versatility including the degradation of compounds that other peroxidases are not able to oxidize directly, versatile peroxidase versatility permits its application in Mn3+-mediated or Mn-independent reactions on both low and high redox-potential aromatic substrates and dyes, versatile peroxidase can be used to reoxidize Mn-containing polyoxometalates, which are efficient oxidizers in paper pulp delignification | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
A260F/R257A | site-directed mutagenesis | Pleurotus eryngii |
R257D | versatile peroxidase activity on Reactive Black 5 is eliminated by the R257D mutation | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H2O2 | the Mn2+-binding site in versatile peroxidase is formed by the side-chains of Glu36, Glu40, and Asp175 located in front of the internal (i.e. more distant from the main haem access-channel) propionate of haem, and connected to the solvent by a narrow access-channel that presents a variable geometry during catalysis | Pleurotus eryngii | Mn3+ + H2O | - |
? | |
additional information | versatile peroxidase is able to oxidize typical substrates of other peroxidases, these ยhybridย properties are due to the coexistence in a single protein of different catalytic sites reminiscent of those present in the other basidiomycete peroxidase families | Pleurotus eryngii | ? | - |
? | |
Reactive Black 5 + H2O2 | versatile peroxidase activity on Reactive Black 5 is eliminated by the R257D mutation | Pleurotus eryngii | ? | - |
? | |
veratryl alcohol + H2O2 + H+ | a solvent-exposed tryptophan is the catalytically-active residue in veratryl alcohol oxidation, initiating an electron transfer pathway to haem | Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
versatile peroxidase | - |
Pleurotus eryngii |