Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.16 extracted from

  • Ruiz-Duenas, F.J.; Morales, M.; Perez-Boada, M.; Choinowski, T.; Martinez, M.J.; Piontek, K.; Martinez, A.T.
    Manganese oxidation site in Pleurotus eryngii versatile peroxidase: a site-directed mutagenesis, kinetic, and crystallographic study (2007), Biochemistry, 46, 66-77.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pleurotus eryngii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, crystal structures of untreated versatile peroxidase (immediately after expression in Escherichia coli and in vitro reconstitution), native versatile peroxidase (treated with Mn2+), D175A variant, and wild-type verstile peroxidase (from Pleurotus eryngii culture) Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
A173R kcat/KM for Mn2+ is 1.4fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.4fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.3fold higher than wild-type value Pleurotus eryngii
D175A kcat/KM for Mn2+ is 842fold lower than wild-type value, kcat/Km for veratryl alcohol is3.2 fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.8fold higher than wild-type value Pleurotus eryngii
E36A kcat/KM for Mn2+ is 258fold lower than wild-type value, kcat/Km for veratryl alcohol is identical to wild-type value, kcat/Km for Reactive Black 5 is 1.2fold higher than wild-type value Pleurotus eryngii
E36A/E40A kcat/KM for Mn2+ is 16000fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.1fold higher than wild-type value Pleurotus eryngii
E36A/E40A/D175A kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is nearly identical to wild-type value, kcat/Km for Reactive Black 5 is 2fold higher than wild-type value Pleurotus eryngii
E36A/E40A/D175A/P327ter kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.6fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value Pleurotus eryngii
E36D kcat/KM for Mn2+ is 77fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 3.5fold higher than wild-type value Pleurotus eryngii
E40A kcat/KM for Mn2+ is 1231fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.2fold lower than wild-type value, kcat/Km for Reactive Black 5 is nearly identical to wild-type value Pleurotus eryngii
E40D kcat/KM for Mn2+ is 54fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.417
-
Mn2+ 25°C, pH 5, mutant A173R Pleurotus eryngii
4.91
-
Mn2+ 25°C, pH 5, mutant E40D Pleurotus eryngii
4.98
-
Mn2+ 25°C, pH 5, mutant E36D Pleurotus eryngii
11.26
-
Mn2+ 25°C, pH 5, mutant E40A Pleurotus eryngii
13.84
-
Mn2+ 25°C, pH 5, mutant E36A Pleurotus eryngii
16.1
-
Mn2+ 25°C, pH 5, mutant D175A Pleurotus eryngii
46.86
-
Mn2+ 25°C, pH 5, mutant E36A/E40A Pleurotus eryngii
69.97
-
Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A/P327ter Pleurotus eryngii
76.4
-
Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A Pleurotus eryngii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme contains Ca2+ Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753 versatile peroxidase VPL2 precursor
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O
-
?
Reactive Black 5 + H2O2
-
Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Pleurotus eryngii veratraldehyde + H2O
-
?

Synonyms

Synonyms Comment Organism
versatile peroxidase VPL2 precursor
-
Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A Pleurotus eryngii
2
-
Mn2+ 25°C, pH 5, mutant E36A/E40A/D175A/P327ter Pleurotus eryngii
5
-
Mn2+ 25°C, pH 5, mutant E36A/E40A Pleurotus eryngii
15
-
Mn2+ 25°C, pH 5, mutant E40A Pleurotus eryngii
32
-
Mn2+ 25°C, pH 5, mutant D175A Pleurotus eryngii
85
-
Mn2+ 25°C, pH 5, mutant E36A Pleurotus eryngii
103
-
Mn2+ 25°C, pH 5, mutant E36D Pleurotus eryngii
145
-
Mn2+ 25°C, pH 5, mutant E40D Pleurotus eryngii
467
-
Mn2+ 25°C, pH 5, mutant A173R Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
heme enzyme contains heme Pleurotus eryngii