Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pleurotus eryngii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structures of untreated versatile peroxidase (immediately after expression in Escherichia coli and in vitro reconstitution), native versatile peroxidase (treated with Mn2+), D175A variant, and wild-type verstile peroxidase (from Pleurotus eryngii culture) | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
A173R | kcat/KM for Mn2+ is 1.4fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.4fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.3fold higher than wild-type value | Pleurotus eryngii |
D175A | kcat/KM for Mn2+ is 842fold lower than wild-type value, kcat/Km for veratryl alcohol is3.2 fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.8fold higher than wild-type value | Pleurotus eryngii |
E36A | kcat/KM for Mn2+ is 258fold lower than wild-type value, kcat/Km for veratryl alcohol is identical to wild-type value, kcat/Km for Reactive Black 5 is 1.2fold higher than wild-type value | Pleurotus eryngii |
E36A/E40A | kcat/KM for Mn2+ is 16000fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.1fold higher than wild-type value | Pleurotus eryngii |
E36A/E40A/D175A | kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is nearly identical to wild-type value, kcat/Km for Reactive Black 5 is 2fold higher than wild-type value | Pleurotus eryngii |
E36A/E40A/D175A/P327ter | kcat for Mn2+ is 149fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.6fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value | Pleurotus eryngii |
E36D | kcat/KM for Mn2+ is 77fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold higher than wild-type value, kcat/Km for Reactive Black 5 is 3.5fold higher than wild-type value | Pleurotus eryngii |
E40A | kcat/KM for Mn2+ is 1231fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.2fold lower than wild-type value, kcat/Km for Reactive Black 5 is nearly identical to wild-type value | Pleurotus eryngii |
E40D | kcat/KM for Mn2+ is 54fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.3fold lower than wild-type value, kcat/Km for Reactive Black 5 is 2.4fold higher than wild-type value | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.417 | - |
Mn2+ | 25°C, pH 5, mutant A173R | Pleurotus eryngii | |
4.91 | - |
Mn2+ | 25°C, pH 5, mutant E40D | Pleurotus eryngii | |
4.98 | - |
Mn2+ | 25°C, pH 5, mutant E36D | Pleurotus eryngii | |
11.26 | - |
Mn2+ | 25°C, pH 5, mutant E40A | Pleurotus eryngii | |
13.84 | - |
Mn2+ | 25°C, pH 5, mutant E36A | Pleurotus eryngii | |
16.1 | - |
Mn2+ | 25°C, pH 5, mutant D175A | Pleurotus eryngii | |
46.86 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A | Pleurotus eryngii | |
69.97 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A/D175A/P327ter | Pleurotus eryngii | |
76.4 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A/D175A | Pleurotus eryngii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme contains Ca2+ | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | versatile peroxidase VPL2 precursor | - |
Purification (Comment) | Organism |
---|---|
recombinant | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | - |
? | |
Reactive Black 5 + H2O2 | - |
Pleurotus eryngii | oxidized Reactive Black 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
versatile peroxidase VPL2 precursor | - |
Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A/D175A | Pleurotus eryngii | |
2 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A/D175A/P327ter | Pleurotus eryngii | |
5 | - |
Mn2+ | 25°C, pH 5, mutant E36A/E40A | Pleurotus eryngii | |
15 | - |
Mn2+ | 25°C, pH 5, mutant E40A | Pleurotus eryngii | |
32 | - |
Mn2+ | 25°C, pH 5, mutant D175A | Pleurotus eryngii | |
85 | - |
Mn2+ | 25°C, pH 5, mutant E36A | Pleurotus eryngii | |
103 | - |
Mn2+ | 25°C, pH 5, mutant E36D | Pleurotus eryngii | |
145 | - |
Mn2+ | 25°C, pH 5, mutant E40D | Pleurotus eryngii | |
467 | - |
Mn2+ | 25°C, pH 5, mutant A173R | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme contains heme | Pleurotus eryngii |