Literature summary for 1.11.1.14 extracted from

Tuisel, H.; Sinclair, R.; Bumpus, J.A.; Ashbaugh, W.; Brock, B.J.; Aust, S.D.
Lignin peroxidase H2 from Phanerochaete chrysosporium purification, characterization and stability to temperature and pH (1990), Arch. Biochem. Biophys., 279, 158-166 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
3-Amino-1,2,4-triazole	-	Phanerodontia chrysosporium
azide	-	Phanerodontia chrysosporium
cyanide	-	Phanerodontia chrysosporium
EDTA	-	Phanerodontia chrysosporium
H2O2	incubation of lignin peroxidase H2 with excess H2O2 in the absence of another reducing substrate results in partial and irreversible inactivation of the enzyme	Phanerodontia chrysosporium
additional information	not inhibitory: chloride	Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	H2O2	pH 4.5, 25°C	Phanerodontia chrysosporium
0.047	-	H2O2	pH 3.5, 25°C	Phanerodontia chrysosporium
0.091	-	H2O2	pH 2.5, 25°C	Phanerodontia chrysosporium
0.132	-	veratryl alcohol	pH 4.5, 25°C	Phanerodontia chrysosporium
0.167	-	veratryl alcohol	pH 3.5, 25°C	Phanerodontia chrysosporium
0.21	-	veratryl alcohol	pH 2.5, 25°C	Phanerodontia chrysosporium

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	P11542	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
veratryl alcohol + H2O2	-	Phanerodontia chrysosporium	veratraldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 38500, SDS-PAGE	Phanerodontia chrysosporium

Synonyms

Synonyms	Comment	Organism
Glg4	-	Phanerodontia chrysosporium
ligninase H2	-	Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	substrate veratryl alcohol, pH 2.5	Phanerodontia chrysosporium
45	-	substrate veratryl alcohol, pH 3.5	Phanerodontia chrysosporium
45	60	substrate veratryl alcohol, pH 4.5	Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	21 h, pH 4.0-6.5, 80% residual activity	Phanerodontia chrysosporium
50	-	stable for 48 h up to 50°C	Phanerodontia chrysosporium
60	-	6 h, complete loss of activity	Phanerodontia chrysosporium
70	-	min, complete loss of activity	Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5	-	substrate veratryl alcohol, 25°C	Phanerodontia chrysosporium
3	-	substrate veratryl alcohol, 35°C	Phanerodontia chrysosporium
3.5	-	substrate veratryl alcohol, 45°C	Phanerodontia chrysosporium

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	7.5	-	Phanerodontia chrysosporium

pI Value

Organism	Comment	pI Value Maximum	pI Value
Phanerodontia chrysosporium	isoelectric focusing	-	4.3

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Release 2023.1 (January 2023)