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Literature summary for 1.11.1.13 extracted from

  • Cheng, X.; Jia, R.; Li, P.; Tu, S.; Zhu, Q.; Tang, W.; Li, X.
    Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme (2007), Enzyme Microb. Technol., 41, 258-264.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Schizophyllum sp.
EDTA partial inhibition Schizophyllum sp.
Fe2+ no significant effect at 0.1 mM, inhibition at 1.0 mM concentration Schizophyllum sp.
Fe3+ no significant effect at 0.1 mM, inhibition at 1.0 mM concentration Schizophyllum sp.
Na+ no significant effect at 1.0 mM, inhibition at 10 mM concentration Schizophyllum sp.
NaN3 partial inhibition Schizophyllum sp.
SDS partial inhibition Schizophyllum sp.
Thiourea partial inhibition Schizophyllum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
H2O2 30°C, pH 4.5 Schizophyllum sp.
0.0131
-
guaiacol 30°C, pH 4.5 Schizophyllum sp.
0.0352
-
Mn2+ 30°C, pH 4.5 Schizophyllum sp.
0.0478
-
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) 30°C, pH 4.5 Schizophyllum sp.
0.1229
-
2,6-dimethoxyphenol 30°C, pH 4.5 Schizophyllum sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Schizophyllum sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48700
-
x * 48700, SDS-PAGE Schizophyllum sp.

Organism

Organism UniProt Comment Textmining
Schizophyllum sp.
-
-
-
Schizophyllum sp. F17
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 18% carbohydrate Schizophyllum sp.

Purification (Commentary)

Purification (Comment) Organism
-
Schizophyllum sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Schizophyllum sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) + H2O2
-
Schizophyllum sp. ?
-
?
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) + H2O2
-
Schizophyllum sp. F17 ?
-
?
2,6-dimethoxyphenol + H2O2
-
Schizophyllum sp. ?
-
?
2,6-dimethoxyphenol + H2O2
-
Schizophyllum sp. F17 ?
-
?
guaiacol + H2O2
-
Schizophyllum sp. ?
-
?
guaiacol + H2O2
-
Schizophyllum sp. F17 ?
-
?
Mn2+ + H+ + H2O2
-
Schizophyllum sp. Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2
-
Schizophyllum sp. F17 Mn3+ + H2O
-
?
additional information the enzyme efficiently decolorized azo dyes such as Congo Red, Orange G and Orange IV Schizophyllum sp. ?
-
?
additional information the enzyme efficiently decolorized azo dyes such as Congo Red, Orange G and Orange IV Schizophyllum sp. F17 ?
-
?

Subunits

Subunits Comment Organism
? x * 48700, SDS-PAGE Schizophyllum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
oxidation of 2,6-dimethoxyphenol Schizophyllum sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable for more than 1 months Schizophyllum sp.
25
-
half-life: 24 h Schizophyllum sp.
30
-
half-life: 13 h Schizophyllum sp.
35
-
half-life: 6 h Schizophyllum sp.
40
-
half-life: 1 h Schizophyllum sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.3
-
guaiacol 30°C, pH 4.5 Schizophyllum sp.
69
-
2,6-dimethoxyphenol 30°C, pH 4.5 Schizophyllum sp.
87.1
-
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) 30°C, pH 4.5 Schizophyllum sp.
373
-
H2O2 30°C, pH 4.5 Schizophyllum sp.
479.7
-
Mn2+ 30°C, pH 4.5 Schizophyllum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
oxidation of 2,6-dimethoxyphenol Schizophyllum sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
half-life: 19 h Schizophyllum sp.
5
-
half-life: 23 h Schizophyllum sp.
6
-
half-life: 15 h Schizophyllum sp.
7
-
half-life: 9 h Schizophyllum sp.

pI Value

Organism Comment pI Value Maximum pI Value
Schizophyllum sp. isoelectric focusing
-
3.6