KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
H2O2 | 0.1 mM Mn2+, MnP1 | Agrocybe praecox | |
0.002 | - |
H2O2 | 0.1 mM Mn2+, MnP1 | Stropharia coronilla | |
0.012 | - |
Mn2+ | 0.1 mM H2O2, MnP1 | Stropharia coronilla | |
0.017 | - |
Mn2+ | 0.1 mM H2O2, MnP1 | Agrocybe praecox | |
0.667 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | 0.1 mM H2O2, Mn2+-independent activity, MnP1 | Agrocybe praecox |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Agrocybe praecox | - |
- |
extracellular | - |
Stropharia coronilla | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | MnP is induced by Mn2+ | Agrocybe praecox | |
additional information | MnP is induced by Mn2+ | Stropharia coronilla |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
42000 | - |
x * 42000, SDS-PAGE | Agrocybe praecox |
43000 | - |
x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Agrocybe praecox | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Stropharia coronilla | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Stropharia coronilla TM 47-1 | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Agrocybe praecox TM 70.84 | important component of lignin degradation system | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrocybe praecox | - |
2 isoenzymes | - |
Agrocybe praecox TM 70.84 | - |
2 isoenzymes | - |
Stropharia coronilla | - |
3 isoenzymes: Mn2+-inducible MnP1 and MnP3, partly constitutive MnP2 | - |
Stropharia coronilla TM 47-1 | - |
3 isoenzymes: Mn2+-inducible MnP1 and MnP3, partly constitutive MnP2 | - |
Purification (Comment) | Organism |
---|---|
partial purification | Agrocybe praecox |
partial purification, MnP2 and MnP3 are not separated | Stropharia coronilla |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
128 | - |
- |
Agrocybe praecox |
692 | - |
- |
Stropharia coronilla |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+ | - |
Agrocybe praecox | ? | - |
r | |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+ | - |
Agrocybe praecox TM 70.84 | ? | - |
r | |
Mn2+ + H+ + H2O2 | - |
Agrocybe praecox | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Stropharia coronilla | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Agrocybe praecox | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Stropharia coronilla | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Stropharia coronilla TM 47-1 | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Stropharia coronilla TM 47-1 | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Agrocybe praecox TM 70.84 | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Agrocybe praecox TM 70.84 | Mn3+ + H2O | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Agrocybe praecox | ? | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Agrocybe praecox TM 70.84 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Agrocybe praecox |
? | x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Agrocybe praecox |
25 | - |
assay at | Stropharia coronilla |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
MnP1: pI 6.3-7.1, MnP2: 3.5-3.7, MnP3: 5.1 | Stropharia coronilla |
additional information | - |
2 isoenzymes: pI 6.3-7.0 | Agrocybe praecox |