Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium | Pleurotus ostreatus | |
additional information | strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium | Pleurotus pulmonarius | |
additional information | strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium | Pleurotus eryngii | |
additional information | strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium | Lentinus sajor-caju |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | 25% inhibition at 0.5 mM, 75% inhibition at 1 mM | Pleurotus eryngii | |
Mn2+ | partial inhibition of oxidation of NAD(P)H at the beginning of the reaction, NADH: 0.1 mM Mn2+ causes 97% inhibition of MP1 and 72% inhibition of MP2 | Pleurotus eryngii | |
additional information | Mn2+ inhibits MnP synthesis | Pleurotus eryngii | |
Sodium metavanadate | partially inhibits only peroxidase activity against veratryl alcohol | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
vanillylideneacetone | 0.1 mM Mn2+, 0.1 mM H2O2 | Pleurotus eryngii | |
0.006 | 0.01 | H2O2 | 0.1 mM Mn2+ | Pleurotus eryngii | |
0.01 | - |
2,6-dimethoxyphenol | 0.1 mM Mn2+, 0.1 mM H2O2 | Pleurotus eryngii | |
0.015 | - |
Mn2+ | 0.1 mM 2,6-dimethoxyphenol, 0.1 mM H2O2 | Pleurotus eryngii | |
0.02 | - |
Mn2+ | 0.1 mM H2O2 | Pleurotus eryngii | |
0.06 | 0.07 | NADH | - |
Pleurotus eryngii | |
0.16 | 0.25 | 2,6-dimethoxyphenol | 0.1 mM H2O2 | Pleurotus eryngii | |
3 | 3.5 | veratryl alcohol | 0.1 mM H2O2 | Pleurotus eryngii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Pleurotus ostreatus |
43000 | - |
x * 43000, SDS-PAGE | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Pleurotus ostreatus | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Pleurotus eryngii | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Pleurotus ostreatus CBS 411.71 | involved in lignin-degradation | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus sajor-caju | - |
white rot fungus, strain MUCL 29757 | - |
Pleurotus eryngii | - |
white rot fungus, strain CBS 613.91, 2 isoenzymes: MnP1 and MnP2 | - |
Pleurotus ostreatus | - |
- |
- |
Pleurotus ostreatus CBS 411.71 | - |
- |
- |
Pleurotus pulmonarius | - |
white rot fungus, strain CBS 507.85 | - |
Oxidation Stability | Organism |
---|---|
higher resistance to H2O2 than MnP from Phanerochaete chrysosporium | Pleurotus eryngii |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pleurotus ostreatus |
glycoprotein | MP1: up to 5% carbohydrate, MP2: up to 7% carbohydrate | Pleurotus eryngii |
Purification (Comment) | Organism |
---|---|
25fold purification | Pleurotus eryngii |
MnP1 and MnP2 | Pleurotus eryngii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | - |
MP2 | Pleurotus eryngii |
187 | - |
MP1 | Pleurotus eryngii |
Storage Stability | Organism |
---|---|
-20°C, stable | Pleurotus eryngii |
4°C, 72 h, stable | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 + H+ | - |
Pleurotus eryngii | coerulignone + H2O | - |
r | |
4-(4-hydroxy-3-methoxy-phenyl)-2-butanone + H2O2 | - |
Pleurotus eryngii | 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-butan-2-one + 4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one + 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-3-buten-2-one + 3-(3-oxo-butyl)-hexa-2,4-dienedioic acid-1-methyl ester | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus pulmonarius | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Lentinus sajor-caju | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | Mn3+ oxidizes vanillylideneacetone | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | Mn3+ oxidizes phenol red | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus ostreatus | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus eryngii | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus CBS 411.71 | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus ostreatus CBS 411.71 | Mn3+ + H2O | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Pleurotus ostreatus | ? | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Pleurotus eryngii | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Pleurotus ostreatus | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Pleurotus eryngii | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity against phenolic substrates, e.g. phenol red | Pleurotus eryngii | ? | - |
? | |
additional information | Mn2+-dependent oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Pleurotus eryngii | ? | - |
? | |
additional information | Mn2+-independent oxidase activity on NAD(P)H | Pleurotus ostreatus | ? | - |
? | |
additional information | Mn2+-independent oxidase activity on NAD(P)H | Pleurotus eryngii | ? | - |
? | |
additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Pleurotus ostreatus CBS 411.71 | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Pleurotus ostreatus CBS 411.71 | ? | - |
? | |
additional information | Mn2+-independent oxidase activity on NAD(P)H | Pleurotus ostreatus CBS 411.71 | ? | - |
? | |
NADH + H2O2 + H+ | - |
Pleurotus eryngii | ? | - |
r | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus eryngii | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Pleurotus ostreatus |
? | x * 43000, SDS-PAGE | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
additional information | - |
2 isozymes: pI 3.8 and 3.7 | Pleurotus eryngii |
3 | - |
oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ | Pleurotus ostreatus |
3 | - |
oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ | Pleurotus eryngii |
3.5 | - |
oxidation of NAD(P)H | Pleurotus ostreatus |
3.5 | - |
oxidation of NAD(P)H | Pleurotus eryngii |
4 | - |
- |
Pleurotus ostreatus |
4 | - |
oxidation of 2,6-dimethoxyphenol in presence of Mn2+ | Pleurotus eryngii |
5 | - |
oxidation of Mn2+ | Pleurotus ostreatus |
5 | - |
oxidation of Mn2+ | Pleurotus eryngii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | oxidation of veratryl alcohol decreases more than 95% when the pH is increased from 3 to 4.5 and nearly absent at pH 5 | Pleurotus eryngii |
3 | 3.5 | pH 3.5: decrease of 95% of oxidation of Mn2+, pH 3: nearly no oxidation of Mn2+ | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pleurotus ostreatus | |
heme | one mol heme per mol of enzyme | Pleurotus eryngii |