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Literature summary for 1.11.1.13 extracted from

  • Martinez, M.J.; Ruiz-Duenas, F.J.; Guillen, F.; Martinez, A.T.
    Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii (1996), Eur. J. Biochem., 237, 424-432.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus ostreatus
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus pulmonarius
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus eryngii
additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Lentinus sajor-caju

Inhibitors

Inhibitors Comment Organism Structure
H2O2 25% inhibition at 0.5 mM, 75% inhibition at 1 mM Pleurotus eryngii
Mn2+ partial inhibition of oxidation of NAD(P)H at the beginning of the reaction, NADH: 0.1 mM Mn2+ causes 97% inhibition of MP1 and 72% inhibition of MP2 Pleurotus eryngii
additional information Mn2+ inhibits MnP synthesis Pleurotus eryngii
Sodium metavanadate partially inhibits only peroxidase activity against veratryl alcohol Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
vanillylideneacetone 0.1 mM Mn2+, 0.1 mM H2O2 Pleurotus eryngii
0.006 0.01 H2O2 0.1 mM Mn2+ Pleurotus eryngii
0.01
-
2,6-dimethoxyphenol 0.1 mM Mn2+, 0.1 mM H2O2 Pleurotus eryngii
0.015
-
Mn2+ 0.1 mM 2,6-dimethoxyphenol, 0.1 mM H2O2 Pleurotus eryngii
0.02
-
Mn2+ 0.1 mM H2O2 Pleurotus eryngii
0.06 0.07 NADH
-
Pleurotus eryngii
0.16 0.25 2,6-dimethoxyphenol 0.1 mM H2O2 Pleurotus eryngii
3 3.5 veratryl alcohol 0.1 mM H2O2 Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Pleurotus ostreatus
43000
-
x * 43000, SDS-PAGE Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Pleurotus ostreatus involved in lignin-degradation Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2 Pleurotus eryngii involved in lignin-degradation Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2 Pleurotus ostreatus CBS 411.71 involved in lignin-degradation Mn3+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Lentinus sajor-caju
-
white rot fungus, strain MUCL 29757
-
Pleurotus eryngii
-
white rot fungus, strain CBS 613.91, 2 isoenzymes: MnP1 and MnP2
-
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus CBS 411.71
-
-
-
Pleurotus pulmonarius
-
white rot fungus, strain CBS 507.85
-

Oxidation Stability

Oxidation Stability Organism
higher resistance to H2O2 than MnP from Phanerochaete chrysosporium Pleurotus eryngii

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Pleurotus ostreatus
glycoprotein MP1: up to 5% carbohydrate, MP2: up to 7% carbohydrate Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
25fold purification Pleurotus eryngii
MnP1 and MnP2 Pleurotus eryngii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
MP2 Pleurotus eryngii
187
-
MP1 Pleurotus eryngii

Storage Stability

Storage Stability Organism
-20°C, stable Pleurotus eryngii
4°C, 72 h, stable Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2 + H+
-
Pleurotus eryngii coerulignone + H2O
-
r
4-(4-hydroxy-3-methoxy-phenyl)-2-butanone + H2O2
-
Pleurotus eryngii 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-butan-2-one + 4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one + 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-3-buten-2-one + 3-(3-oxo-butyl)-hexa-2,4-dienedioic acid-1-methyl ester
-
?
Mn2+ + H+ + H2O2
-
Pleurotus pulmonarius Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2
-
Lentinus sajor-caju Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes vanillylideneacetone ?
Mn2+ + H+ + H2O2
-
Pleurotus ostreatus Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes phenol red ?
Mn2+ + H+ + H2O2
-
Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes a variety of phenols ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus eryngii Mn3+ + H2O
-
?
Mn2+ + H+ + H2O2
-
Pleurotus ostreatus CBS 411.71 Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus CBS 411.71 Mn3+ + H2O
-
?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus ?
-
?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus eryngii ?
-
?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus ?
-
?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus eryngii ?
-
?
additional information Mn2+-independent peroxidase activity against phenolic substrates, e.g. phenol red Pleurotus eryngii ?
-
?
additional information Mn2+-dependent oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus eryngii ?
-
?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus ?
-
?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus eryngii ?
-
?
additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus CBS 411.71 ?
-
?
additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus CBS 411.71 ?
-
?
additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus CBS 411.71 ?
-
?
NADH + H2O2 + H+
-
Pleurotus eryngii ?
-
r
veratryl alcohol + H2O2 + H+
-
Pleurotus eryngii ?
-
r

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Pleurotus ostreatus
? x * 43000, SDS-PAGE Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
Pleurotus ostreatus
additional information
-
2 isozymes: pI 3.8 and 3.7 Pleurotus eryngii
3
-
oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus ostreatus
3
-
oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus eryngii
3.5
-
oxidation of NAD(P)H Pleurotus ostreatus
3.5
-
oxidation of NAD(P)H Pleurotus eryngii
4
-
-
Pleurotus ostreatus
4
-
oxidation of 2,6-dimethoxyphenol in presence of Mn2+ Pleurotus eryngii
5
-
oxidation of Mn2+ Pleurotus ostreatus
5
-
oxidation of Mn2+ Pleurotus eryngii

pH Range

pH Minimum pH Maximum Comment Organism
3 5 oxidation of veratryl alcohol decreases more than 95% when the pH is increased from 3 to 4.5 and nearly absent at pH 5 Pleurotus eryngii
3 3.5 pH 3.5: decrease of 95% of oxidation of Mn2+, pH 3: nearly no oxidation of Mn2+ Pleurotus eryngii

Cofactor

Cofactor Comment Organism Structure
heme
-
Pleurotus ostreatus
heme one mol heme per mol of enzyme Pleurotus eryngii