Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | less activation than by lactate or malonate | Phanerodontia chrysosporium | |
H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
Lactate | activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential | Phanerodontia chrysosporium | |
malonate | stabilizes Mn3+ at a relatively high redox potential and facilitate oxidation of organic substrates | Phanerodontia chrysosporium | |
oxalate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium | |
oxalate | less activation than by lactate or malonate | Phanerodontia chrysosporium |
Application | Comment | Organism |
---|---|---|
environmental protection | thiol-mediated degradation of dimeric model compounds and of polymeric lignin by MnP has potential applications in the degradation of industrial lignins | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
cDNA sequence encoding a MnP isoenzyme is determined | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
- |
Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | Mn3+ is produced under lignolytic conditions | ? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium OGC101 | important component of lignin degradation system | Mn3+ + H2O | Mn3+ is produced under lignolytic conditions | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Phanerodontia chrysosporium OGC101 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
isoenzyme 1 | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ is produced under lignolytic conditions | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ is produced under lignolytic conditions | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.9 | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.1 | 6.1 | veratryl alcohol oxidation, activity increases with increasing pH | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Phanerodontia chrysosporium | |
heme | heme protein | Phanerodontia chrysosporium |