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Literature summary for 1.11.1.12 extracted from
- Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus (2010), Comp. Biochem. Physiol. B, 155, 54-61.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the baculovirus expression vector system, using the Autographa californica nucleopolyhedrovirus and the Sf9 insect cell line, is used to produce recombinant Bi-PHGPx proteins, the cDNA fragment containing the full-length open reading frame is inserted into the transfer vector pBAC1 | Bombus ignitus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002353 | - |
phosphatidylcholine hydroperoxide | apparent KM | Bombus ignitus |  |
| 0.0008656 | - |
H2O2 | apparent KM | Bombus ignitus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | - |
Bombus ignitus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
determined by SDS-PAGE | Bombus ignitus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | Bombus ignitus | - |
glutathione disulfide + lipid + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombus ignitus | C3VVL8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a HisTrap column | Bombus ignitus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Bombus ignitus | - |
| fat body | extraction of genomic DNA | Bombus ignitus | - |
| hemolymph | - |
Bombus ignitus | - |
| midgut | - |
Bombus ignitus | - |
| muscle | - |
Bombus ignitus | - |
| whole body | cDNA library | Bombus ignitus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + a lipid hydroperoxide | - |
Bombus ignitus | glutathione disulfide + lipid + H2O | - |
? | |
| glutathione + H2O2 | activity assay | Bombus ignitus | ? | - |
? | |
| glutathione + phosphatidylcholine hydroperoxide | activity assay | Bombus ignitus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bi-PHGPx | - |
Bombus ignitus |
| GPx4 | - |
Bombus ignitus |
| PHGPX | - |
Bombus ignitus |
| phospholipid-hydroperoxide glutathione peroxidase | - |
Bombus ignitus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bombus ignitus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
thermal stability shows that the activity is significantly decreased when the temperature is higher than 40°C | Bombus ignitus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bombus ignitus |
| 7.5 | - |
activity assay | Bombus ignitus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Bombus ignitus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Bombus ignitus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bombus ignitus | Bi-PHGPx transcripts are up-regulated by stresses, such as wounding, H2O2 exposure, external temperature shock, and starvation | up |
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