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Literature summary for 1.11.1.11 extracted from

  • Hitomi, S.; Kokabu, S.; Matsumoto, K.; Shoji, Y.; Ujihara, I.; Ono, K.
    Expression of ascorbate peroxidase derived from Cyanidioschyzon merolae in mammalian cells (2020), In Vivo, 34, 2437-2441 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene APX, stable ectopic expression of His-V5-tagged or c-Myc-/GFP-tagged cAPX protein from a CMV-V5-His plasmid in mouse fibroblast-like cell line C3H10T1/2. Confirmation of cell transfection with this plasmid is performed by western blotting using anti-Myc antibody and by fluorescence microscopy. Method, overview Cyanidioschyzon merolae

Protein Variants

Protein Variants Comment Organism
additional information expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular antioxidative capacity. Heat and H2O2 stimulation results in ROS production. cAPX-expressing cells are more tolerant to oxidative stress induced by heat, H2O2, and acid stimulations than control cells lacking cAPX Cyanidioschyzon merolae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in the heme group Cyanidioschyzon merolae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+ Cyanidioschyzon merolae
-
L-ascorbate + L-dehydroascorbate + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Cyanidioschyzon merolae C9K1X1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
Cyanidioschyzon merolae L-ascorbate + L-dehydroascorbate + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
APX
-
Cyanidioschyzon merolae
ascorbate peroxidase
-
Cyanidioschyzon merolae
cAPX
-
Cyanidioschyzon merolae

Cofactor

Cofactor Comment Organism Structure
heme
-
Cyanidioschyzon merolae

General Information

General Information Comment Organism
physiological function the ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments (42°C, pH 2.5), has greater anti-oxidative capacity than similar peroxidases occurring in other plants. Cyanidioschyzon merolae-derived APX (cAPX) expressed in mammalian cells increases cellular antioxidative capacity. Heat and H2O2 stimulation results in ROS production. cAPX-expressing cells are more tolerant to oxidative stress induced by heat, H2O2, and acid stimulations than control cells lacking cAPX Cyanidioschyzon merolae