Cloned (Comment) | Organism |
---|---|
gene APX, stable ectopic expression of His-V5-tagged or c-Myc-/GFP-tagged cAPX protein from a CMV-V5-His plasmid in mouse fibroblast-like cell line C3H10T1/2. Confirmation of cell transfection with this plasmid is performed by western blotting using anti-Myc antibody and by fluorescence microscopy. Method, overview | Cyanidioschyzon merolae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular antioxidative capacity. Heat and H2O2 stimulation results in ROS production. cAPX-expressing cells are more tolerant to oxidative stress induced by heat, H2O2, and acid stimulations than control cells lacking cAPX | Cyanidioschyzon merolae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the heme group | Cyanidioschyzon merolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | Cyanidioschyzon merolae | - |
L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanidioschyzon merolae | C9K1X1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | - |
Cyanidioschyzon merolae | L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APX | - |
Cyanidioschyzon merolae |
ascorbate peroxidase | - |
Cyanidioschyzon merolae |
cAPX | - |
Cyanidioschyzon merolae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cyanidioschyzon merolae |
General Information | Comment | Organism |
---|---|---|
physiological function | the ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments (42°C, pH 2.5), has greater anti-oxidative capacity than similar peroxidases occurring in other plants. Cyanidioschyzon merolae-derived APX (cAPX) expressed in mammalian cells increases cellular antioxidative capacity. Heat and H2O2 stimulation results in ROS production. cAPX-expressing cells are more tolerant to oxidative stress induced by heat, H2O2, and acid stimulations than control cells lacking cAPX | Cyanidioschyzon merolae |