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Literature summary for 1.11.1.11 extracted from

  • Cao, S.; Du, X.; Li, L.; Liu, Y.; Zhang, L.; Pan, X.; Li, Y.; Li, H.; Lu, H.
    Overexpression of Populus tomentosa cytosolic ascorbate peroxidase enhances abiotic stress tolerance in tobacco plants (2017), Russ. J. Plant Physiol., 64, 224-234 .
No PubMed abstract available
Search for more literature for 1.11.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Populus tomentosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 L-ascorbate pH 7.0, temperature not specified in the publication Populus tomentosa
3.43
-
 H2O2 pH 7.0, temperature not specified in the publication Populus tomentosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Populus tomentosa 5829
-

Organism

Organism UniProt Comment Textmining
Populus tomentosa U5RZT9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression levels are higher in leaves than in roots or stems Populus tomentosa
-
root expression levels are higher in leaves than in roots or stems Populus tomentosa
-
stem expression levels are higher in leaves than in roots or stems Populus tomentosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
 Populus tomentosa L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 33240, calculated, x * 33000, SDS-PAGE Populus tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Populus tomentosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.7
-
 H2O2 pH 7.0, temperature not specified in the publication Populus tomentosa
49.6
-
 L-ascorbate pH 7.0, temperature not specified in the publication Populus tomentosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Populus tomentosa

pH Range

pH Minimum pH Maximum Comment Organism
4
-
 80% of maximum activity Populus tomentosa
7
-
 25% of maximum activity Populus tomentosa

General Information

General Information Comment Organism
physiological function transgenic tobacco plants overexpressing Apx show no significant difference in morphology under normal conditions. The transgenic plants are more resistant to drought, salt and oxidative stress conditions and show decreased H2O2 levels, increased ascorbate consumption, an increase in the NADP to NADPH ratio, and higher Apx activity Populus tomentosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.5
-
 L-ascorbate pH 7.0, temperature not specified in the publication Populus tomentosa
59.5
-
 H2O2 pH 7.0, temperature not specified in the publication Populus tomentosa