Application | Comment | Organism |
---|---|---|
agriculture | the transgenic plants show enhanced tolerance to oxidative stress, salt and drought, PpAPX does not play a significant role under normal growing conditions, but do ameliorate oxidative injury under abiotic stress, the Ad29 promoter shoul be used as an inducible promoter in transgenic works | Populus tomentosa |
Cloned (Comment) | Organism |
---|---|
into the pBI121 binary vector for a Agrobacterium tumefaciens-mediated tobacco leaf disc transformation, a CaMV 35S promoter and a rd29A promoter driven construct is used | Populus tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Populus tomentosa | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | Populus tomentosa | - |
dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | Q5S1V5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | - |
Populus tomentosa | dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peroxisomal ascorbate peroxidase | - |
Populus tomentosa |
PpAPX | - |
Populus tomentosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
activity assay | Populus tomentosa |
Organism | Comment | Expression |
---|---|---|
Populus tomentosa | APX activity in the 35S-PpAPX transgenic lines increases by 50% compared to the activity in the control lines, activity is 80% higher in the leaves in response to drought or salt stresses, the PpAPX transcript level is very low under normal growing conditions in rd29Ap-PpAPX plants, but clearly increased under drought stress | up |