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Literature summary for 1.11.1.11 extracted from
- Leishmania major ascorbate peroxidase overexpression protects cells against reactive oxygen species-mediated cardiolipin oxidation (2008), Free Radic. Biol. Med., 45, 1520-1529.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Leishmania major |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pXG-B2863 for transfection of Leishmania major cells | Leishmania major |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Leishmania major | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial intermembrane space | - |
Leishmania major | 5758 | - |
| mitochondrion | - |
Leishmania major | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
determined by SDS-PAGE and Western blot analysis, LmAPX in the intermembrane space of the mitochondrial inner membrane is appr. 3.6 kDa shorter than the full-length gene | Leishmania major |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | Leishmania major | - |
dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | Leishmania major 5ASKH | - |
dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania major | - |
- |
- |
| Leishmania major 5ASKH | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mitochondria are prepared and fractionated | Leishmania major |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrorhodamine 123 + H2O2 | assay, peroxidase substrate | Leishmania major | ? | - |
? | |
| dihydrorhodamine 123 + H2O2 | assay, peroxidase substrate | Leishmania major 5ASKH | ? | - |
? | |
| L-ascorbate + H2O2 | - |
Leishmania major | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Leishmania major 5ASKH | dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ascorbate peroxidase | - |
Leishmania major |
| LmAPX | - |
Leishmania major |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
peroxidase assay | Leishmania major |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Leishmania major | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Leishmania major | expression of LmAPX is increased when cells are treated with exogenous H2O2 | up |
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Release 2023.1 (January 2023)
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