Cloned (Comment) | Organism |
---|---|
GhAPX1 is expressed in Escherichia coli | Gossypium hirsutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
H2O2 | - |
Gossypium hirsutum | |
0.43 | - |
L-ascorbate | - |
Gossypium hirsutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gossypium hirsutum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, calculated from sequence | Gossypium hirsutum |
31500 | - |
2 * 31500, His-tag fused enzyme, SDS-PAGE | Gossypium hirsutum |
64200 | - |
gel filtration | Gossypium hirsutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gossypium hirsutum | GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | A7KIX5 | L. cv. Xuzhou 142 | - |
Purification (Comment) | Organism |
---|---|
- |
Gossypium hirsutum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotton fibre | GhAPX1 is highly expressed in wild-type 5-day postanthesis fibres with much lower transcript levels in the fuzzless-lintless mutant ovules. GhAPX1 expression is upregulated in response to an increase in cellular H2O2 and ethylene | Gossypium hirsutum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + H2O2 | about 20% of the activity with L-ascorbate | Gossypium hirsutum | ? | - |
? | |
guaiacol + H2O2 | about 30% of the activity with L-ascorbate | Gossypium hirsutum | ? | - |
? | |
L-ascorbate + H2O2 | - |
Gossypium hirsutum | dehydroascorbate + H2O | - |
? | |
additional information | GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development | Gossypium hirsutum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, calculated from sequence | Gossypium hirsutum |
dimer | 2 * 31500, His-tag fused enzyme, SDS-PAGE | Gossypium hirsutum |
Synonyms | Comment | Organism |
---|---|---|
GhAPX1 | - |
Gossypium hirsutum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.71 | - |
H2O2 | - |
Gossypium hirsutum | |
260 | - |
L-ascorbate | - |
Gossypium hirsutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
oxidation of ascorbate | Gossypium hirsutum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity | Gossypium hirsutum |