Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.11 extracted from

  • Li, H.B.; Qin, Y.M.; Pang, Y.; Song, W.Q.; Mei, W.Q.; Zhu, Y.X.
    A cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development (2007), New Phytol., 175, 462-471.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
GhAPX1 is expressed in Escherichia coli Gossypium hirsutum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
H2O2
-
Gossypium hirsutum
0.43
-
L-ascorbate
-
Gossypium hirsutum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Gossypium hirsutum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, calculated from sequence Gossypium hirsutum
31500
-
2 * 31500, His-tag fused enzyme, SDS-PAGE Gossypium hirsutum
64200
-
gel filtration Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gossypium hirsutum GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development ?
-
?

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum A7KIX5 L. cv. Xuzhou 142
-

Purification (Commentary)

Purification (Comment) Organism
-
Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
cotton fibre GhAPX1 is highly expressed in wild-type 5-day postanthesis fibres with much lower transcript levels in the fuzzless-lintless mutant ovules. GhAPX1 expression is upregulated in response to an increase in cellular H2O2 and ethylene Gossypium hirsutum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSSG + H2O2 about 20% of the activity with L-ascorbate Gossypium hirsutum ?
-
?
guaiacol + H2O2 about 30% of the activity with L-ascorbate Gossypium hirsutum ?
-
?
L-ascorbate + H2O2
-
Gossypium hirsutum dehydroascorbate + H2O
-
?
additional information GhAPX1 is involved in hydrogen peroxide homeostasis during cotton fibre development Gossypium hirsutum ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, calculated from sequence Gossypium hirsutum
dimer 2 * 31500, His-tag fused enzyme, SDS-PAGE Gossypium hirsutum

Synonyms

Synonyms Comment Organism
GhAPX1
-
Gossypium hirsutum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46.71
-
H2O2
-
Gossypium hirsutum
260
-
L-ascorbate
-
Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
oxidation of ascorbate Gossypium hirsutum

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5: about 50% of maximal activity, pH 7.5: about 50% of maximal activity Gossypium hirsutum