Protein Variants | Comment | Organism |
---|---|---|
C126A | kcat/KM for L-ascorbate is 1.4fold higher than wild-type enzyme. kcat/Km for H2O2 is 1.7fold lower than wild-type enzyme | Nicotiana tabacum |
C26S | kcat/KM for L-ascorbate is 1.8fold lower than wild-type enzyme. kcat/Km for H2O2 is 3.1fold lower than wild-type enzyme | Nicotiana tabacum |
C26S/C126A | kcat/KM for L-ascorbate is 1.3fold lower than wild-type enzyme. kcat/Km for H2O2 is 2.5fold lower than wild-type enzyme | Nicotiana tabacum |
C26S/W35F/C126A | kcat/KM for L-ascorbate is 1.6fold than wild-type enzyme. kcat/Km for H2O2 is 3.4fold lower than wild-type enzyme. Mutant shows increased tolerance to H2O2 (retains 50% of the initial activity after H2O2 treatment for 3 min) compared to wild-type enzyme (half-time of inactivation is less than 10 sec) | Nicotiana tabacum |
W35F | kcat/KM for L-ascorbate is 2.6fold higher than wild-type enzyme. kcat/Km for H2O2 is 2.3fold lower than wild-type enzyme | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | wild-type enzyme has a half-time of inactivation of less than 10 sec. Triple mutant C26S/W35F/C126A retains 50% of the initial activity after H2O2 treatment for 3 min | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
H2O2 | wild-type enzyme | Nicotiana tabacum | |
0.0343 | - |
H2O2 | mutant enzyme C126A | Nicotiana tabacum | |
0.0537 | - |
H2O2 | mutant enzyme C26S/C126A | Nicotiana tabacum | |
0.0606 | - |
H2O2 | mutant enzyme C26S | Nicotiana tabacum | |
0.0939 | - |
H2O2 | mutant enzyme W35F | Nicotiana tabacum | |
0.136 | - |
H2O2 | mutant enzyme C26S/W35F/C126A | Nicotiana tabacum | |
0.167 | - |
L-ascorbate | mutant enzyme W35F | Nicotiana tabacum | |
0.19 | - |
L-ascorbate | mutant enzyme C26S/W35F/C126A | Nicotiana tabacum | |
0.198 | - |
L-ascorbate | mutant enzyme C126A | Nicotiana tabacum | |
0.395 | - |
L-ascorbate | wild-type enzyme | Nicotiana tabacum | |
0.506 | - |
L-ascorbate | mutant enzyme C26S/C126A | Nicotiana tabacum | |
0.566 | - |
L-ascorbate | mutant enzyme C26S | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma | Nicotiana tabacum | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q9TNL9 | recombinant enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | - |
Nicotiana tabacum | dehydroascorbate + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1580 | - |
H2O2 | mutant enzyme C26S | Nicotiana tabacum | |
1630 | - |
H2O2 | mutant enzyme C126A | Nicotiana tabacum | |
1710 | - |
L-ascorbate | mutant enzyme C126A | Nicotiana tabacum | |
1730 | - |
H2O2 | mutant enzyme C26S/C126A | Nicotiana tabacum | |
1800 | - |
H2O2 | wild-type enzyme | Nicotiana tabacum | |
1910 | - |
L-ascorbate | mutant enzyme C26S/W35F/C126A | Nicotiana tabacum | |
2040 | - |
L-ascorbate | mutant enzyme C26S | Nicotiana tabacum | |
2400 | - |
L-ascorbate | mutant enzyme C26S/C126A | Nicotiana tabacum | |
2510 | - |
L-ascorbate | wild-type enzyme | Nicotiana tabacum | |
2833 | - |
L-ascorbate | mutant enzyme W35F | Nicotiana tabacum | |
3280 | - |
H2O2 | mutant enzyme C26S/W35F/C126A | Nicotiana tabacum | |
3410 | - |
H2O2 | mutant enzyme W35F | Nicotiana tabacum |