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Literature summary for 1.11.1.11 extracted from

  • Kitajima, S.; Kitamura, M.; Koja, N.
    Triple mutation of Cys26, Trp35, and Cys126 in stromal ascorbate peroxidase confers H2O2 tolerance comparable to that of the cytosolic isoform (2008), Biochem. Biophys. Res. Commun., 372, 918-923.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C126A kcat/KM for L-ascorbate is 1.4fold higher than wild-type enzyme. kcat/Km for H2O2 is 1.7fold lower than wild-type enzyme Nicotiana tabacum
C26S kcat/KM for L-ascorbate is 1.8fold lower than wild-type enzyme. kcat/Km for H2O2 is 3.1fold lower than wild-type enzyme Nicotiana tabacum
C26S/C126A kcat/KM for L-ascorbate is 1.3fold lower than wild-type enzyme. kcat/Km for H2O2 is 2.5fold lower than wild-type enzyme Nicotiana tabacum
C26S/W35F/C126A kcat/KM for L-ascorbate is 1.6fold than wild-type enzyme. kcat/Km for H2O2 is 3.4fold lower than wild-type enzyme. Mutant shows increased tolerance to H2O2 (retains 50% of the initial activity after H2O2 treatment for 3 min) compared to wild-type enzyme (half-time of inactivation is less than 10 sec) Nicotiana tabacum
W35F kcat/KM for L-ascorbate is 2.6fold higher than wild-type enzyme. kcat/Km for H2O2 is 2.3fold lower than wild-type enzyme Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
H2O2 wild-type enzyme has a half-time of inactivation of less than 10 sec. Triple mutant C26S/W35F/C126A retains 50% of the initial activity after H2O2 treatment for 3 min Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
H2O2 wild-type enzyme Nicotiana tabacum
0.0343
-
H2O2 mutant enzyme C126A Nicotiana tabacum
0.0537
-
H2O2 mutant enzyme C26S/C126A Nicotiana tabacum
0.0606
-
H2O2 mutant enzyme C26S Nicotiana tabacum
0.0939
-
H2O2 mutant enzyme W35F Nicotiana tabacum
0.136
-
H2O2 mutant enzyme C26S/W35F/C126A Nicotiana tabacum
0.167
-
L-ascorbate mutant enzyme W35F Nicotiana tabacum
0.19
-
L-ascorbate mutant enzyme C26S/W35F/C126A Nicotiana tabacum
0.198
-
L-ascorbate mutant enzyme C126A Nicotiana tabacum
0.395
-
L-ascorbate wild-type enzyme Nicotiana tabacum
0.506
-
L-ascorbate mutant enzyme C26S/C126A Nicotiana tabacum
0.566
-
L-ascorbate mutant enzyme C26S Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma Nicotiana tabacum 9507
-

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q9TNL9 recombinant enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ascorbate + H2O2
-
Nicotiana tabacum dehydroascorbate + H2O
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1580
-
H2O2 mutant enzyme C26S Nicotiana tabacum
1630
-
H2O2 mutant enzyme C126A Nicotiana tabacum
1710
-
L-ascorbate mutant enzyme C126A Nicotiana tabacum
1730
-
H2O2 mutant enzyme C26S/C126A Nicotiana tabacum
1800
-
H2O2 wild-type enzyme Nicotiana tabacum
1910
-
L-ascorbate mutant enzyme C26S/W35F/C126A Nicotiana tabacum
2040
-
L-ascorbate mutant enzyme C26S Nicotiana tabacum
2400
-
L-ascorbate mutant enzyme C26S/C126A Nicotiana tabacum
2510
-
L-ascorbate wild-type enzyme Nicotiana tabacum
2833
-
L-ascorbate mutant enzyme W35F Nicotiana tabacum
3280
-
H2O2 mutant enzyme C26S/W35F/C126A Nicotiana tabacum
3410
-
H2O2 mutant enzyme W35F Nicotiana tabacum