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Literature summary for 1.11.1.11 extracted from

  • Lad, L.; Mewies, M.; Basran, J.; Scrutton, N.S.; Raven, E.L.
    Role of histidine 42 in ascorbate peroxidase. Kinetic analysis of the H42A and H42E variants (2002), Eur. J. Biochem., 269, 3182-3192.
    View publication on PubMed
Search for more literature for 1.11.1.11

Protein Variants

Protein Variants Comment Organism
H42A inactive, partial recovery of activity by addition of exogenous imidazoles Pisum sativum
H42E decrease in kcat- and Km-value Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 L-ascorbate pH 7.0, 25°C, mutant H42E Pisum sativum
0.41
-
 L-ascorbate pH 7.0, 25°C, wild-type Pisum sativum
2.9
-
 guaiacol pH 7.0, 25°C, mutant H42E Pisum sativum
12.3
-
 guaiacol pH 7.0, 25°C, wild-type Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pisum sativum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron predominantly five-coordinate high-spin iron Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 L-ascorbate + H2O2 + 2 H+ = 2 monodehydroascorbate + 2 H2O mechanism involves accumulation of a transient enzyme intermediate Pisum sativum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
 Pisum sativum ? + H2O
-
 ?
L-ascorbate + H2O2
-
 Pisum sativum ? + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 guaiacol pH 7.0, 25°C, mutant H42E Pisum sativum
3.9
-
 L-ascorbate pH 7.0, 25°C, mutant H42E Pisum sativum
66
-
 guaiacol pH 7.0, 25°C, wild-type Pisum sativum
248
-
 L-ascorbate pH 7.0, 25°C, wild-type Pisum sativum