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Literature summary for 1.11.1.11 extracted from

  • De Leonardis, S.; Dipierro, N.; Dipierro, S.
    Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria (2000), Plant Physiol. Biochem., 38, 773-779.
No PubMed abstract available
Search for more literature for 1.11.1.11

General Stability

General Stability Organism
the enzyme is completely inactivated within 30 s in ascorbate-depleted medium under anaerobic conditions Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
cysteine 100% inhibition at 5 mM Solanum tuberosum
iodoacetamide 30% inhibition at 1 mM, 65% inhibition at 5 mM Solanum tuberosum
KCN 100% inhibition at 0.5 mM Solanum tuberosum
Mersalyl 58% inhibition at 0.005 mM, 100% inhibition at 0.05 mM Solanum tuberosum
N-ethylmaleimide 33% inhibition at 0.05 mM, 28% inhibition at 0.5 mM Solanum tuberosum
NaN3 80% inhibition at 1 mM Solanum tuberosum
p-hydroxymercuribenzoate 43% inhibition at 0.005 mM, 100% inhibition at 0.05 mM Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 ascorbate
-
 Solanum tuberosum
0.08
-
 H2O2
-
 Solanum tuberosum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inside, either bound to the inner membrane or in the matrix Solanum tuberosum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ascorbate + H2O2 Solanum tuberosum role of the mitochondrial enzyme in the scanvenging of toxic oxygen species inside potato tuber mitochondria dehydroascorbate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using lysed mitochondria, and column chromatography on DEAE-Sephacel, Sephadex G-75, DE-52 and hydroxylapatite Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
 purified enzyme Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2 the activity is lower than with L-ascorbate Solanum tuberosum ?
-
 ?
L-ascorbate + H2O2 highly specific for Solanum tuberosum dehydroascorbate + H2O
-
 ?
L-ascorbate + H2O2 role of the mitochondrial enzyme in the scanvenging of toxic oxygen species inside potato tuber mitochondria Solanum tuberosum dehydroascorbate + 2 H2O
-
 ?
pyrogallol + H2O2 the activity is lower than with L-ascorbate Solanum tuberosum 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Solanum tuberosum