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Literature summary for 1.11.1.11 extracted from
- Purification and characterization of cytosolic ascorbate peroxidase from komatsuna (Brassica rapa) (1996), Plant Sci., 120, 11-18.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCN | complete inhibition at 0.05 mM | Brassica rapa |  |
| NaN3 | complete inhibition at 1 mM | Brassica rapa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
H2O2 | - |
Brassica rapa | |
| 0.402 | - |
ascorbate | - |
Brassica rapa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast stroma | - |
Brassica rapa | 9570 | - |
| cytosol | - |
Brassica rapa | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Brassica rapa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | Brassica rapa | - |
dehydroascorbate + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using column chromatography on DEAE-Sephacel, ammonium sulfate treatment and column chromatography on butyl-Toyopearl and TSK G3000SW | Brassica rapa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Brassica rapa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56 | - |
purified enzyme | Brassica rapa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytochrome c + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
| glutathione + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
| guaiacol + H2O2 | no reaction | Brassica rapa | ? | - |
? | |
| L-ascorbate + H2O2 | L-ascorbate is the most effective natural electron donor | Brassica rapa | dehydroascorbate + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Brassica rapa | dehydroascorbate + 2 H2O | - |
? | |
| additional information | the cytosolic enzyme exhibits no activity with: glutathione, cytochrome c and NAD(P)H | Brassica rapa | ? | - |
? | |
| NADPH + H2O2 | no activity | Brassica rapa | ? + H2O | - |
? | |
| pyrogallol + H2O2 | 2.5-fold higher rate than that of L-ascorbate | Brassica rapa | 3-hydroxybenzo-1,2-quinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Brassica rapa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Brassica rapa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
complete loss of activity | Brassica rapa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
assay at | Brassica rapa |
| 6.5 | - |
- |
Brassica rapa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Brassica rapa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Brassica rapa | |
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