General Stability | Organism |
---|---|
the catalytic efficiency of free CPO is decreased about 1.6fold upon immobilization. The conjugated-CPO activity on the poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane remains almost the same as the original activity after 9 cycles. After that, a steady decrease in chlorination capability of the conjugated-CPO is observed, and this loss reaches about 27% after 25 cycles of batch operation | Leptoxyphium fumago |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0261 | - |
Monochlorodimedon | free enzyme, in citrate buffer (50 mM, pH 3.0) at 25°C | Leptoxyphium fumago | |
0.0277 | - |
Monochlorodimedon | bio-conjugated enzyme, in citrate buffer (50 mM, pH 3.0) at 25°C | Leptoxyphium fumago |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptoxyphium fumago | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, conjugated-CPO in citrate buffer (50 mM, pH 3.0), 8 weeks, the conjugated-CPO preserves its initial activity more than 60% | Leptoxyphium fumago |
4°C, free and conjugated-CPO in citrate buffer (50 mM, pH 3.0), 4 weeks, the activity loss of the conjugated-CPO is 7% while the free enzyme loses its initial activity about 83% | Leptoxyphium fumago |
4°C, free enzyme in citrate buffer (50 mM, pH 3.0), 6 weeks, the free enzyme loses all its activity | Leptoxyphium fumago |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
monochlorodimedon + Cl- + H2O2 | - |
Leptoxyphium fumago | dichlorodimedon + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPO | - |
Leptoxyphium fumago |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane | Leptoxyphium fumago |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane | Leptoxyphium fumago |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | at 45°C, the conjugated-CPO preserved loses about 7% of its initial activity whereas the free enzyme loses about 34% of its initial activity during a 120 min incubation period. At 55°C, the conjugated-CPO and free CPO retain their activity about to a level of 53% and 11%, respectively | Leptoxyphium fumago |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane | Leptoxyphium fumago |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 5 | free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane | Leptoxyphium fumago |