Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.10 extracted from

  • Bayramoglu, G.; Altintas, B.; Yilmaz, M.; Arica, M.Y.
    Immobilization of chloroperoxidase onto highly hydrophilic polyethylene chains via bio-conjugation: Catalytic properties and stabilities (2010), Bioresour. Technol., 102, 475-482.
    View publication on PubMed

General Stability

General Stability Organism
the catalytic efficiency of free CPO is decreased about 1.6fold upon immobilization. The conjugated-CPO activity on the poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane remains almost the same as the original activity after 9 cycles. After that, a steady decrease in chlorination capability of the conjugated-CPO is observed, and this loss reaches about 27% after 25 cycles of batch operation Leptoxyphium fumago

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0261
-
Monochlorodimedon free enzyme, in citrate buffer (50 mM, pH 3.0) at 25°C Leptoxyphium fumago
0.0277
-
Monochlorodimedon bio-conjugated enzyme, in citrate buffer (50 mM, pH 3.0) at 25°C Leptoxyphium fumago

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-

Storage Stability

Storage Stability Organism
4°C, conjugated-CPO in citrate buffer (50 mM, pH 3.0), 8 weeks, the conjugated-CPO preserves its initial activity more than 60% Leptoxyphium fumago
4°C, free and conjugated-CPO in citrate buffer (50 mM, pH 3.0), 4 weeks, the activity loss of the conjugated-CPO is 7% while the free enzyme loses its initial activity about 83% Leptoxyphium fumago
4°C, free enzyme in citrate buffer (50 mM, pH 3.0), 6 weeks, the free enzyme loses all its activity Leptoxyphium fumago

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monochlorodimedon + Cl- + H2O2
-
Leptoxyphium fumago dichlorodimedon + H2O
-
?

Synonyms

Synonyms Comment Organism
CPO
-
Leptoxyphium fumago

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane Leptoxyphium fumago

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane Leptoxyphium fumago

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 at 45°C, the conjugated-CPO preserved loses about 7% of its initial activity whereas the free enzyme loses about 34% of its initial activity during a 120 min incubation period. At 55°C, the conjugated-CPO and free CPO retain their activity about to a level of 53% and 11%, respectively Leptoxyphium fumago

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane Leptoxyphium fumago

pH Range

pH Minimum pH Maximum Comment Organism
2 5 free and bio-conjugated-CPO poly(hydroxypropyl)methacrylateco-poly(ethylene glycol)-methacrylate-3 membrane Leptoxyphium fumago