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Literature summary for 1.11.1.10 extracted from

  • Ayala, M.; Horjales, E.; Pickard, M.A.; Vazquez-Duhalt, R.
    Cross-linked crystals of chloroperoxidase (2002), Biochem. Biophys. Res. Commun., 295, 828-831.
    View publication on PubMed
Search for more literature for 1.11.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, crystals are modified by several cross-linkers, but only glutaraldehyde is able to produce catalytically active and insoluble crystals. Although the cross-linked crystals are catalytically active, they show lower specific activity than the soluble enzyme Leptoxyphium fumago

General Stability

General Stability Organism
crystal crosslinking with glutaraldehyde yields a chloroperoxidase preparation with enhanced thermal resistance compared to soluble enzyme Leptoxyphium fumago

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thianthrene + H2O2 + Cl-
-
 Leptoxyphium fumago ?
-
 ?
thioanisole + H2O2 + Cl-
-
 Leptoxyphium fumago ?
-
 ?

Synonyms

Synonyms Comment Organism
chloroperoxidase
-
 Leptoxyphium fumago

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 crystal crosslinking with glutaraldehyde yields a chloroperoxidase preparation with enhanced thermal resistance compared to soluble enzyme Leptoxyphium fumago
50
-
 1 h, 50% loss of activity of crystals cross-linked with glutaraldehyde and of soluble enzyme Leptoxyphium fumago
60
-
 1 h, about 10% loss of activity of crystals cross-linked with glutaraldehyde and more than 90% loss of activity of soluble enzyme Leptoxyphium fumago
70
-
 1 h, about 10% loss of activity of crystals cross-linked with glutaraldehyde and more than 95% loss of activity of soluble enzyme Leptoxyphium fumago
80
-
 1 h, about 95% loss of activity of crystals cross-linked with glutaraldehyde and complete than 90% loss of activity of soluble enzyme Leptoxyphium fumago