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Literature summary for 1.10.3.6 extracted from
- Rifamycin B oxidase from Monocillium spp., a new type of diphenol oxidase (1983), FEBS Lett., 151, 36-40.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | slight activation | Monocillium spp. |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | apparent inhibition | Monocillium spp. | |
| NADPH | apparent inhibition | Monocillium spp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rifamycin B + O2 | Monocillium spp. | reverse reaction of rifamycin B biosynthesis | rifamycin O + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monocillium spp. | - |
ATCC 20621 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ion-exchange, gel filtration | Monocillium spp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| rifamycin B + O2 = rifamycin O + H2O2 | acting on diphenols and related substances as electron donors and on oxygen as an electron acceptor | Monocillium spp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.19 | - |
- |
Monocillium spp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-hydroquinone + O2 | rapid oxidation | Monocillium spp. | ? | - |
? | |
| pyrogallol + ? | - |
Monocillium spp. | ? | - |
? | |
| rifamycin B + O2 | rapid oxidation | Monocillium spp. | rifamycin O + H2O2 | - |
? | |
| rifamycin B + O2 | reverse reaction of rifamycin B biosynthesis | Monocillium spp. | rifamycin O + H2O2 | - |
? | |
| rifamycin SV + ? | - |
Monocillium spp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | assay at | Monocillium spp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Monocillium spp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme contains no flavins, heme, Cu2+ or other metal ions | Monocillium spp. |
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Release 2023.1 (January 2023)
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